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. 2019 Apr-Jun;14(2):204-213.

Comprehensive Study of Phenotypic and Growth Rate Features of Blastocystis Subtypes 1-3 and 6 in Symptomatic and Asymptomatic Subjects

Seyed Ahmad Karamati  1 , Hamed Mirjalali  2 , Maryam Niyyati  1 , Tahereh Rezaei Riabi  1 , Abbas Yadegar  2 , Hamid Asadzadeh Aghdaei  3 , Ali Haghighi  1 , Seyyed Javad Seyyed Tabaei  1 , Mohammad Reza Zali  4
Affiliations

Comprehensive Study of Phenotypic and Growth Rate Features of Blastocystis Subtypes 1-3 and 6 in Symptomatic and Asymptomatic Subjects

Seyed Ahmad Karamati et al. Iran J Parasitol. 2019 Apr-Jun.

Abstract

Background: The present study aimed to assess the grouping of subtypes 1-3 and 6 of Blastocystis according to the size and generation time of the parasite among the symptomatic and asymptomatic subjects.

Methods: Blastocystis subtypes 1-3 and 6 isolated from symptomatic and asymptomatic subjects and were cultivated in DMEM medium. In order to assess inter- and intra-subtype variation in size, all the isolates were measured using morphometric criteria. Generation time was calculated using approximately 1×104 Blastocystis, which were cultivated in DMEM, every 24h for 4 days.

Results: All subtypes had 5 to 185 μm diameter range. The smallest size was attributed to ST1, followed by ST6 and ST2. ST3 showed the most variable size and phenotypes compared with the other three subtypes. Furthermore, amoeboid forms and parasite clumping were only seen in ST3-S (symptomatic subjects). Generation time analysis showed that the number of ST1 isolated from symptomatic and asymptomatic subjects peaked higher than the other subtypes.

Conclusion: This is the first study discussing inter-intra-size, phenotype and generation time variations among 4 common subtypes of Blastocystis. Accordingly, ST3 was largest subtype and showed most diversities in both size and phenotype, while ST1 was smallest subtype with lowest intra-subtype variation.

Keywords: Blastocystis; Generation time; Phenotypic variation; Subtypes.

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Conflict of interest statement

Conflict of interest The authors declare that they have no conflict of interest.

Figures

Fig. 1:
Fig. 1:
Variation size, clumping and amoeboid form of Blastocystis subtypes. (S: symptomatic subjects and As: asymptomatic subjects)
Fig. 2:
Fig. 2:
Phenotypic & size variation of Blastocystis ST3 isolated from symptomatic subjects
Fig. 3:
Fig. 3:
Variation size between Blastocystis subtypes (ST1 –3 and 6). ST3 isolated from symptomatic subjects showed significantly higher size range in comparison with other groups apart from ST3 isolated from asymptomatic subjects
Fig. 4:
Fig. 4:
Growth profile of Blastocystis isolates (ST1–3 and 6) cultured in DMEM medium in four times (24–96 h)
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