Molecular characterization of clinical and environmental carbapenem resistant Acinetobacter baumannii isolates in a hospital of the Eastern Region of Saudi Arabia

Abstract

Background: Environmental and clinical carbapenem-resistant Acinetobacter baumannii (CRAb) isolated in a hospital of the Eastern Region of Saudi Arabia were compared to assess the potential environmental contamination by this pathogen.

Methods: Frequent-hand-touch surfaces of intensive care (ICU), medical (MW), and surgical (SW) units were randomly sampled for a month-long period, and the CRAb identified were compared to clinical isolates of the same period by PFGE and bla_OXA-51-like gene sequencing. Carbapenemase and ribosomal methylase genes, ISAba1 link to bla_OXA51-like or to bla_OXA-23, respectively were detected by PCR.

Results: CRAb was identified from 35.5% of surfaces. All environmental and clinical isolates were multi- or extremely drug resistant. PFGE of all clinical (n=21) and selected environmental (n=30) isolates identified a singleton and four clusters, all of which included both clinical and environmental isolates. In the two largest clusters isolates carried bla_OXA-66, ISAba1-linked bla_OXA-23, and were from the ICU, MW and the male SW. Isolates of the female SW carried bla_OXA-69, ISAba1-linked bla_OXA-23 and bla_GES-11. A pair of clinical and environmental CRAb from the Male SW harboured bla_NDM-1 in addition to ISAba1-linked bla_OXA-94.

Conclusion: A worrying level of environmental contamination, often by CRAb belonging to international clones, was revealed, highlighting the importance of environmental hygiene.

Keywords: Acinetobacter baumannii; Environmental contamination; Molecular typing; Multidrug resistance.