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. 2019 Sep 1;20(9):2831-2840.
doi: 10.31557/APJCP.2019.20.9.2831.

Cytotoxicity Profiling of Annona Squamosa in Cancer Cell Lines

Biba Vikas  1 , Sukumaran Anil  2 , P Remani  1
Affiliations

Cytotoxicity Profiling of Annona Squamosa in Cancer Cell Lines

Biba Vikas et al. Asian Pac J Cancer Prev. .

Abstract

Objective: In the study our aim was to evaluate the cytotoxic activity of different solvent extracts of Annona squamosa seeds. Methods and materials: The four extracts used were petroleum ether, chloroform, ethyl acetate and methanol were tested using cytotoxicity assays. Results: Among the four extracts tested petroleum ether showed maximum cytotoxicity against a panel of cancer cell lines such as nasopharyngeal cancer (KB) cells, lung cancer (A-549) cells, breast cancer (MCF- 7) cells, leukemic (K-562) cells and inhibited the growth of murine cancer cells such as Dalton’s lymphoma ascites (DLA) and Ehrlich ascites carcinoma (EAC). Conclusion: Petroleum ether extract of Annona squamosa seeds showed cytotoxicity towards a panel of cancer cells meanwhile non-significant cytotoxicity towards normal cells.

Keywords: Annona squamosa; Anticancer; Cytotoxicity.

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Conflict of interest statement

The authors declare no conflict of interest. The project was funded by Indian Council of Medical research

Figures

Figure 1
Figure 1
Effects of Different Solvent Extracts on DLA Cells Trypan Blue Dye Exclusion Method
Figure 2
Figure 2
Effects of Different Solvent Extracts on K-562 Cells Trypan Blue Dye Exclusion Method
Figure 3
Figure 3
Cytotoxicity of Annona Squamosa Seed Extracts on KB Cells at 24 Hours by Mttassay
Figure 4
Figure 4
Cytotoxicity of Annona squamosa Seed Extracts on A-549 Cells at 24 Hours by MTT Assay
Figure 5
Figure 5
Cytotoxicity of Annona Squamosa Seed Extracts on MCF-7 Cells at 24 Hours by MTT Assay
Figure 6
Figure 6
Cytotoxicity of Annona Squamosa Seed Extracts on K-562 Cells at 24 Hours by MTT Assay
Figure 7
Figure 7
Cytotoxicity of Annona Squamosa Seed Extracts DLA at 24 Hours by MTT Assay
Figure 8
Figure 8
Cytotoxicity of Annona Squamosa Seed Extracts EAC at 24 Hours by MTT Assay
Figure 9
Figure 9
Cytotoxicity of Annona Squamosa Seed Extracts KB at 24 Hours by MTT Assay
Figure 10
Figure 10
Cytotoxicity of ASPE on KB Cells at 12,24 & 48 Hour by MTT Assay
Figure 11
Figure 11
Cytotoxicity of ASPE on A-549 Cells at 12,24 & 48 Hours by MTT Assay
Figure 12
Figure 12
Cytotoxicity of ASPE on MCF-7 Cells at 12,24 & 48 Hours by MTT Assay
Figure 13
Figure 13
Cytotoxicity of ASPE on K-562 Cells at 12,24 & 48 Hours by MTT Assay
Figure 14
Figure 14
Cytotoxicity of ASPE on DLA Cells at 12,24 & 48 Hours by MTT Assay
Figure 15
Figure 15
Cytotoxicity of ASPE on EAC Cells at 12,24 & 48 Hours by MTT Assay
Figure 16
Figure 16
Lymphocyte Viability Assay
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