90-Day repeated oral toxicity test of D-allulose produced from Microbacterium foliorum

Abstract

D-allulose is considered an ideal substitute for sucrose, because it has 70% of the sweetness of sucrose and ultra-low energy. Chemical and biotechnological methods have been developed to produce Dallulose from D-fructose because D-allulose exists in extremely small quantities in nature. In this study, we performed a 90-day repeated oral dose toxicity test on rats using D-allulose produced from Microbacterium foliorum-a non-GMO species isolated from salad ginseng-in dosages of 0, 1250, 2500 and 5000 mg/kg/day. We developed a toxicity determination criterion based on the significant change caused by the administration of the substance to estimate the NOEL, NOAEL, and LOAEL of the substance applied in this study. This test found only minor compound-related changes in both male and female rats in the high dose group and no important compound-related changes. Thus, we determined the NOAEL of Dallulose in both sexes to be 5,000 mg/kg/day. This study's finding of a NOAEL of 5,000 mg/kg/day should ensure that D-allulose produced from Microbacterium foliorum is classified as a safe and ordinary substance.

Keywords: 90-Day toxicity test; D-allulose; D-psicose; No-observed-adverse-effect-level (NOAEL); Repeated dose.