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. 2020 Mar;65(1):250-255.
doi: 10.2478/s11686-019-00129-6. Epub 2019 Sep 30.

First Report of Molecular Characterization of Oochoristica hemidactyli Johri, 1955 (Cestoda, Linstowiidae) from the Common Wall Lizard, Hemidactylus brooki Gray, 1845 (Reptilia: Gekkonidae) Along with Morphological Redescription

Chandni Verma  1 , Anshu Chaudhary  2 , Bindu Sharma  1 , Hridaya Shanker Singh  1
Affiliations

First Report of Molecular Characterization of Oochoristica hemidactyli Johri, 1955 (Cestoda, Linstowiidae) from the Common Wall Lizard, Hemidactylus brooki Gray, 1845 (Reptilia: Gekkonidae) Along with Morphological Redescription

Chandni Verma et al. Acta Parasitol. 2020 Mar.

Abstract

Introduction: During an investigation, a rare cestode species belonging to the genus, Oochoristica were found in Meerut, UP, India, from the intestine of the host, Hemidactylus brookii Gray, 1845 (Reptilia: Gekkonidae). The tapeworms were found to be similar in their morphological characters with Oochoristica hemidactyli Johri, 1955 (Cestoda: Linstowiidae).

Materials and methods: The host Hemidactylus brookii used in this analysis was caught from May to July 2018 from Department of Zoology, Chaudhary Charan Singh University, Meerut (UP), India. Parasites were examined by microscopy. 18S and ITS1-5.8S-ITS2 region of ribosomal RNA were sequenced and analyzed for the molecular study.

Results: This paper provides the first trustworthy confirmation about the occurrence of O. hemidactyli inferred with molecular data using ribosomal 18S and ITS1-5.8S-ITS2 gene cluster DNA sequences. Molecular analysis shows that the studied specimens were clustered separately and did not show a close relationship with any other cestode spp., represented in the GenBank.

Conclusion: This is the foremost molecular characterization of any species of Oochoristica and of O. hemidactyli after 64 years of its original description that contributing to the systematic analysis of the helminthological biodiversity of reptilian hosts. These results provide a reference for future molecular study of species of Oochoristica.

Keywords: 18S; Cestode; Hemidactylus brookii; ITS1-5.8S-ITS2; India; Oochoristica hemidactyli.

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