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. 2019 Sep 10:12:2819-2826.
doi: 10.2147/IDR.S214960. eCollection 2019.

Diversity and frequency of resistance and virulence genes in bla KPC and bla NDM co-producing Klebsiella pneumoniae strains from China

Xin Liu #  1 , Jie Zhang #  1 , Yini Li  1 , Qiuni Shen  1 , Wenting Jiang  1 , Kelei Zhao  2 , Yancheng He  1 , Penggao Dai  1 , Zhihao Nie  1 , Xiyan Xu  1   3 , Yingshun Zhou  1
Affiliations

Diversity and frequency of resistance and virulence genes in bla KPC and bla NDM co-producing Klebsiella pneumoniae strains from China

Xin Liu et al. Infect Drug Resist. .

Abstract

Background: Emergence of bla KPC and bla NDM co-producing Klebsiella pneumoniae strains have led to the limited therapeutic options for clinical treatment. Understanding the diversity and frequency of resistance and virulence genes of these isolates is of great significance.

Purpose: The aim of this study is to research the diversity and frequency of resistance and virulence genes in the bla KPC and bla NDM co-producing Klebsiella pneumoniae strains.

Methods and results: In this study, 117 K. pneumonia strains were isolated from China, and among of which, 24 were found to be bla KPC and bla NDM co-producing with significant resistance against almost all the commonly used antibiotics. Additionally, 4 strains were hypermucoviscous and 8 showed high serum resistance. Overall, bla SHV, bla CTX-M, tetA and sul1 resistance genes found in 100% of the isolates, followed by bla TEM (95.8%), oqxA/B (91.7%), qnrB (87.5%), aac(6')Ib-cr (83.3%), bla DHA (79.2%), rmtB (66.7%), qnrS (54.2%), cat(54.2%), floR (50.0%), sul2 (45.8%) cmlA (20.8%)andbla CMY (8.33%), respectively. What' more, seven bla CTX-M subtypes [bla CTX-M-14 (n=18), bla CTX-M-3(n=11), bla CTX-M-65 (n=4), bla CTX-M-15 (n=3), bla CTX-M-28 (n=2), bla CTX-M-55 (n=2), bla CTX-M-22 (n=1)] and six bla SHV subtypes [bla SHV-12(n=16), bla SHV-11 (n=4), bla SHV-2a(n=1), bla SHV-1(n=1), bla SHV-38(n=1) and bla SHV-28(n=1)] were detected. The frequency of virulence genes was as follows: 100% for entB, ybtS and irp, 95.8% for mrkD, 91.66% for fimH, 79.2% for iutA, 62.5% for iroBCDE, aerobactin and kfu, 66.7% for allS, 45.8% for wcaG, 37.5% for rmpA, 20.8% for pagO and 16.7% for magA.

Conclusion: From this study, we concluded that the bla KPC and bla NDM co-producing Klebsiella pneumoniae strains have a high diversity and frequency of resistance and virulence genes. This study may offer hospitals important information about the control of infections caused by bla KPC and bla NDM co-producing Klebsiella pneumoniae.

Keywords: Klebsiella pneumoniae; blaKPC; blaNDM; resistance genes; virulence factors.

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Conflict of interest statement

The authors declare that there are no conflicts of interest in this work.

Figures

Figure 1
Figure 1
The dendrogram of ERIC-PCR fingerprints and diversity of the ESBLs genotypes. Notes: The dendrogram of ERIC-PCR fingerprints was constructed using the Dice coefficient and the unweighted pair-group method with arithmetic mean (UPGMA) and the diversity of the ESBLs (blaCTX-M group and blaSHV group) genotypes.
See this image and copyright information in PMC

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