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. 2019 Sep;15(5):1247-1253.
doi: 10.5114/aoms.2019.87095. Epub 2019 Aug 8.

Expression of miRNAs-122, -192 and -499 in end stage renal disease associated with acute myocardial infarction

Lobna Abdelsalam  1 , Alshaymaa A Ibrahim  2 , Aliaa Shalaby  2 , Noha Osman  3 , Assem Hashad  4 , Dina Badawy  5 , Hany Elghobary  1 , Eman Amer  6
Affiliations

Expression of miRNAs-122, -192 and -499 in end stage renal disease associated with acute myocardial infarction

Lobna Abdelsalam et al. Arch Med Sci. 2019 Sep.

Abstract

Introduction: New diagnostic tools are needed to accurately detect acute myocardial infarction (AMI) in patients with end stage renal disease (ESRD) presenting with ischemic chest pain. We aimed in this study to investigate circulating miR-122, -192 and -499 expression levels in patients with AMI on top of ESRD and evaluate the potential of these miRNAs as blood-based biomarkers for AMI in patients with ESRD.

Material and methods: The study included 80 ESRD patients without AMI, 80 patients with ESRD associated with AMI and 60 healthy subjects. Assessment of microRNAs was done using SYBR Green based real-time PCR.

Results: Levels of miR-122 were 28-fold and 20-fold higher in controls than in ESRD patients with or without AMI respectively (p < 0.001), while no differences were detected between the two patient groups (p = 0.9). Levels of miR-192 showed a marked increase in ESRD patients with and without AMI compared to the control group (> 500-fold, > 8000-fold respectively, p ≤ 0.001). Patients who developed AMI had lower expression than ESRD patients without AMI (p < 0.001). Non-significant miR-499 elevation was found in ESRD patients without cardiac disease compared to the control group, while highly significant elevation of miR- 499 was demonstrated in ESRD patients who developed AMI compared to other ESRD patients and the control group (> 100-fold, > 350-fold respectively, p = 0.001).

Conclusions: Altered expression of miR-122 and -192 may contribute in pathogenesis of ESRD. MiR-192 and -499 may serve as potential biomarkers for AMI in ESRD. Further studies are needed to correlate these miRNAs with disease progression and outcome.

Keywords: acute myocardial infarction; chronic kidney disease; end stage renal disease; miRNA.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Mean expression of miR-122 (A), miR-192 (B) and miR-499 (C) in the studied groups ESRD – end stage renal disease, AMI – acute myocardial infarction.
Figure 2
Figure 2
Receiver operating characteristic (ROC) curves of the studied miRNAs in differentiation of end stage renal disease with acute myocardial infarction from those without
See this image and copyright information in PMC

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