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Review
. 2019 Sep 13:6:88.
doi: 10.3389/fmolb.2019.00088. eCollection 2019.

Natural and Synthetic Sialylated Glycan Microarrays and Their Applications

Alyssa M McQuillan  1 , Lauren Byrd-Leotis  1   2 , Jamie Heimburg-Molinaro  1 , Richard D Cummings  1
Affiliations
Review

Natural and Synthetic Sialylated Glycan Microarrays and Their Applications

Alyssa M McQuillan et al. Front Mol Biosci. .

Abstract

This focused chapter serves as a short survey of glycan microarrays that are available with sialylated glycans, including both defined and shotgun arrays, their generation, and their utility in studying differential binding interactions to sialylated compounds, highlighting N-glycolyl (Gc) modified sialylated compounds. A brief discussion of binding interactions by lectins, antibodies, and viruses, and their relevance that have been observed with sialylated glycan microarrays is presented, as well as a discussion of cross-comparisons of array platforms and efforts to centralize and standardize the glycan microarray data.

Keywords: Neu5Ac; Neu5Gc; functional glycomics; glycan binding proteins; glycan microarrays; natural glycans; sialylated glycans; synthetic glycans.

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Figures

Figure 1
Figure 1
Influenza A virus binding to CFG array. The strength of IAV binding to each glycan on the CFG array is visualized by the force plot, where bound glycans are linked to the IAV spot (magenta) in the middle and unbound glycans are pushed to the periphery. Neu5Ac and Neu5Gc sialylated glycans are highlighted in blue and green, respectively. The structures corresponding to one example each of Neu5Ac and Neu5Gc terminating glycans are displayed and the binding to each structure is highlighted on the bar chart comparing RFU. Glycan cartoon key: light blue diamond- Neu5Gc, purple diamond- Neu5Ac, yellow circle- Gal, blue square- GlcNAc, green circle- Man, red triangle- Fuc, black circle- linker. Figure produced using the GLycan Array Dashboard (GLAD) program, developed by the Cummings group (Mehta and Cummings, 2019), website- https://glycotoolkit.com/Tools/GLAD/.
Figure 2
Figure 2
General method for creation and use of glycan microarrays from natural materials.
See this image and copyright information in PMC

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