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. 2019 Sep;13(7):682-687.
doi: 10.1049/iet-nbt.2018.5330.

Synthesis of ZnO/Fe3O4/rGO nanocomposites and evaluation of antibacterial activities towards E. coli and S. aureus

Arunima Rajan S  1 , Ahmaduddin Khan  1 , Syed Asrar  2 , Hasan Raza  2 , Raunak Kumar Das  2 , Niroj Kumar Sahu  3
Affiliations

Synthesis of ZnO/Fe3O4/rGO nanocomposites and evaluation of antibacterial activities towards E. coli and S. aureus

Arunima Rajan S et al. IET Nanobiotechnol. 2019 Sep.

Abstract

Antibacterial activity of nanoparticles (NPs) and nanocomposites (NCs) has received wide spread attention in biomedical applications. In this direction, the authors prepared zinc oxide (ZnO), iron oxide (Fe3O4), and their composite including reduced graphene oxide (rGO) by hydrothermal method. The structural and microstructural properties of the synthesised NPs and NCs were investigated by XRD, FT-IR, UV-Vis, TGA, and TEM analysis. PEG-coated ZnO and Fe3O4 form in hexagonal wurtzite and inverse spinel structures, respectively. ZnO forms in rod-shaped (aspect ratio of ∼3) morphology, whereas well-dispersed spherical-shaped morphology of ∼10 nm is observed in Fe3O4 NPs. The ZnO/Fe3O4 composite possesses a homogeneous distribution of above two phases and shows a very good colloidal stability in aqueous solvent. These synthesised particles exhibited varying antibacterial activity against gram-positive strain Staphylococcus aureus (S. aureus) and gram-negative strain Escherichia coli (E. coli). The nanocomposite exhibits a better cidal effect on E. coli when compared to S. aureus when treated with 1 mg/ml concentration. Further, the addition of rGO has intensified the anti-bacterial effect to a much higher extent due to synergistic influence of individual components.

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Figures

Fig. 1
Fig. 1
XRD patterns of NPs and NCs (a) rGO, (b) Fe3 O4, (c) ZnO, (d) Fe3 O4 /ZnO and (e) ZnO/Fe3 O4 /rGO
Fig. 2
Fig. 2
FTIR spectra of NPs and NCs (a) Fe3 O4, (b) ZnO, (c) Fe3 O4 /ZnO and (d) Fe3 O4 /ZnO/rGO
Fig. 3
Fig. 3
UV‐Vis absorption spectra of NPs and NCs (a) Fe3 O4, (b) ZnO, (c) Fe3 O4 /ZnO and (d) ZnO/Fe3 O4 /rGO
Fig. 4
Fig. 4
Weight loss profile of (a) Fe3 O4, (b) ZnO, (c) Fe3 O4 /ZnO and (d) ZnO/Fe3 O4 /rGO with respect to heating temperature
Fig. 5
Fig. 5
TEM images of (a) Fe3 O4, (b) ZnO NPs, (c) Fe3 O4 /ZnO NC and (d) SAED pattern of Fe3 O4 /ZnO NC
Fig. 6
Fig. 6
Antibacterial activity of NPs and NCs against E. coli when treated with concentration of (a) 1 mg/ml and (b) 2 mg/ml
Fig. 7
Fig. 7
Antibacterial activity of NPs and NCs against S. aureus when treated with concentration of (a) 1 mg/ml and (b) 2 mg/ml
Fig. 8
Fig. 8
Disc diffusion images of nanoparticles on (a) E. coli and (b) S. aureus
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