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. 2019 Oct 1;2019(10).
doi: 10.1101/pdb.prot095430.

Calcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs

Priti Kumar, Arvindhan Nagarajan, Pradeep D Uchil

Calcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs

Priti Kumar et al. Cold Spring Harb Protoc. .

Abstract

This protocol describes a calcium phosphate-mediated transfection method for use with plasmid DNAs and adherent cells. At the end of the protocol is an alternative method for high-efficiency generation of stable transfectants.

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