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. 2019 Oct 2;11(19):8313-8328.
doi: 10.18632/aging.102321. Epub 2019 Oct 2.

LncRNAs down-regulate Myh1, Casr, and Mis18a expression in the Substantia Nigra of aged male rats

Guoliang Zhang  1   2 , Yunxiao Kang  1 , Xu Feng  3 , Rui Cui  2 , Qiqing Guo  1 , Xiaoming Ji  1 , Yuanxiang Huang  4 , Yannan Ma  1 , Shufeng Liu  3 , Geming Shi  1   5   6
Affiliations

LncRNAs down-regulate Myh1, Casr, and Mis18a expression in the Substantia Nigra of aged male rats

Guoliang Zhang et al. Aging (Albany NY). .

Abstract

In this study, we used high-throughput RNA sequencing to identify mRNAs, long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) that are differentially expressed in the Substantia Nigra (SN) of aged and young rats. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to perform functional annotation of mRNAs that were either differentially expressed themselves (DEMs), targeted by differentially expressed lncRNAs (DELs), or the parents of differentially expressed circRNAs (DECs). A total of 112 DEMs, 163 DELs, and 98 DECs were found in the SN of aged rats. The down-regulated lncRNA NONRATT010417.2 targeted the down-regulated mRNA Myh1, while the down-regulated lncRNA NONRATT015586.2 and the up-regulated lncRNAs NONRATT000490.2 and NONRATT007029.2 all targeted the down-regulated mRNAs Casr and Mis18a. Western blots and RT-qPCR revealed that Myh1, Casr, and Mis18a protein and mRNA expression were significantly reduced in aged rats compared to young rats. This study improves our understanding of the transcriptional alterations underlying aging-related changes in the SN and provides a foundation for future studies of associated molecular mechanisms.

Keywords: Substantia Nigra; aged male rats; circular RNAs; long non-coding RNAs; messenger RNAs.

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Conflict of interest statement

CONFLICTS OF INTEREST: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Scatter diagrams (A–C), volcano diagrams (D–F), and hierarchical clustering analysis (G–I) of relative mRNA (A, D, G), lncRNA (B, E, H), and circRNA (C, F, I) expression signals in the SN of 6Mon and 24Mon rats.
Figure 2
Figure 2
The biotype of DELs (A) and DECs (B) in the SN of 6Mon and 24Mon rats.
Figure 3
Figure 3
Functional annotation of DEMs in the SN of 6Mon and 24Mon rats. (A) GO classification, (B) Top 30 GO enrichments, (C) KEGG classifications, (D) Top 30 KEGG pathway enrichments.
Figure 4
Figure 4
Functional annotation of mRNAs targeted by DELs in the SN of 6Mon and 24Mon rats. (A) GO classification, (B) Top 30 GO enrichments, (C) KEGG classifications, (D) Top 30 KEGG pathway enrichments.
Figure 5
Figure 5
Functional annotation of parental mRNAs of DECs in the SN of 6Mon and 24Mon rats. (A) GO classification, (B) Top 30 GO enrichments, (C) KEGG classifications, (D) Top 30 KEGG pathway enrichments.
Figure 6
Figure 6
Functional annotation of DEMs targeted by DELs in the SN of 6Mon and 24Mon rats. (A) GO classification, (B) KEGG pathway analysis showed that Myh1 was responsive to cellular processes and significantly enriched in the tight junction (rno04530).
Figure 7
Figure 7
Western blot and RT-qPCR showing Myh1, Casr, and Miss18a expression in the SN of 6Mon and 24Mon rats. (A) Western blot images, (B) Bar graphs illustrating Myh1, Casr, and Mis18a protein expression, (C) Bar graphs illustrating Myh1, Casr, and Mis18a mRNA expression, *P < 0.01.
See this image and copyright information in PMC

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