Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2019 Nov 22;57(12):e01391-19.
doi: 10.1128/JCM.01391-19. Print 2019 Dec.

Tailoring Antimicrobial Susceptibility Testing to Individual Species of Coagulase-Negative Staphylococci: Next Up, Staphylococcus epidermidis

C Paul Morris  1 , Patricia J Simner  2
Affiliations
Comment

Tailoring Antimicrobial Susceptibility Testing to Individual Species of Coagulase-Negative Staphylococci: Next Up, Staphylococcus epidermidis

C Paul Morris et al. J Clin Microbiol. .

Abstract

Accurate detection of methicillin resistance among staphylococci is vital for patient care. Methicillin resistance is most commonly mediated by acquisition of the mecA gene, which encodes an altered penicillin binding protein, PBP2a. Application of phenotypic methods to detect mecA-mediated beta-lactam resistance in staphylococci is becoming more complex as species-specific differences are identified among coagulase-negative staphylococci (CoNS). Previously, interpretative criteria and antimicrobial susceptibility testing (AST) methods specific to the CoNS group were used to evaluate Staphylococcus epidermidis A manuscript by S. N. Naccache, K. Callan, C.-A. D. Burnham, M. A. Wallace, et al. (J Clin Microbiol 57:e00961-19, 2019, https://doi.org/10.1128/JCM.00961-19) details experiments revealing that S. epidermidis, the most common clinically isolated CoNS, requires tailored use of previously described methods and interpretive criteria to reliably identify the presence of mecA-mediated methicillin resistance.

PubMed Disclaimer

Comment on

References

    1. Huse HK, Miller SA, Chandrasekaran S, Hindler JA, Lawhon SD, Bemis DA, Westblade LF, Humphries RM. 2017. Evaluation of oxacillin and cefoxitin disk diffusion and MIC breakpoints established by the Clinical and Laboratory Standards Institute for detection of mecA-mediated oxacillin resistance in Staphylococcus schleiferi. J Clin Microbiol 56:e01653-17. - PMC - PubMed
    1. Becker K, Heilmann C, Peters G. 2014. Coagulase-negative staphylococci. Clin Microbiol Rev 27:870–926. doi:10.1128/CMR.00109-13. - DOI - PMC - PubMed
    1. Sader HS, Jones RN. 2012. Antimicrobial activity of daptomycin in comparison to glycopeptides and other antimicrobials when tested against numerous species of coagulase-negative Staphylococcus. Diagn Microbiol Infect Dis 73:212–214. doi:10.1016/j.diagmicrobio.2012.02.005. - DOI - PubMed
    1. Sadovskaya I, Vinogradov E, Flahaut S, Kogan G, Jabbouri S. 2005. Extracellular carbohydrate-containing polymers of a model biofilm-producing strain, Staphylococcus epidermidis. Infect Immun 73:3007–3017. doi:10.1128/IAI.73.5.3007-3017.2005. - DOI - PMC - PubMed
    1. Sabaté Brescó M, Harris LG, Thompson K, Stanic B, Morgenstern M, O'Mahony L, Richards RG, Moriarty TF. 2017. Pathogenic mechanisms and host interactions in Staphylococcus epidermidis device-related infection. Front Microbiol 8:1401. doi:10.3389/fmicb.2017.01401. - DOI - PMC - PubMed