Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec 1;98(12):6281-6288.
doi: 10.3382/ps/pez576.

In vitro evaluation of various antimicrobials against field mycoplasma gallisepticum and mycoplasma synoviae isolates in Egypt

Marwa I Abd El-Hamid  1 , Naglaa F S Awad  2 , Yousreya M Hashem  3 , Mahmoud A Abdel-Rahman  4 , Adel M Abdelaziz  5 , Imad A A Mohammed  2 , Usama H Abo-Shama  6
Affiliations

In vitro evaluation of various antimicrobials against field mycoplasma gallisepticum and mycoplasma synoviae isolates in Egypt

Marwa I Abd El-Hamid et al. Poult Sci. .

Abstract

Among many avian mycoplasmas, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are recognized as the main etiological agents of respiratory diseases and infectious synovitis in chickens and turkeys causing tremendous economic losses worldwide. Therefore, proper treatment is promoted for the control of these diseases. This study was the first in Egypt to evaluate the in vitro efficacy of various antimicrobials against field MG and MS isolates recovered from chicken and turkey flocks using both conventional broth microdilution and quantitative real-time polymerase chain reaction assays. Totally, 47 mycoplasma isolates were recovered from 160 collected tracheal samples (29.4%). Of these, 44 MG (27.5%) and 3 MS (1.9%) were identified using conventional and molecular assays. The in vitro susceptibilities of 4 representative mycoplasma field isolates (3 MG and one MS) to 8 antibiotics and 4 essential oils were investigated. The tested isolates showed various susceptibilities to tested antimicrobials. Toldin CRD, followed by clove, cumin, and cinnamon oils were effective against both MG and MS clinical isolates with minimum inhibitory concentration (MIC) values ranging from 0.49 to 15.63 µg/mL. Similarly, tylvalosin was the most active antibiotic against MG and MS isolates with the lowest MIC values (0.015 to 0.03 µg/mL). DNA loads of both MG mgc2 and MS vlhA genes were markedly decreased upon treatment with majority of the tested antimicrobials confirming their effectiveness as was also evaluated by conventional MIC results. In conclusion, Toldin CRD and tylvalosin were found to be the most effective antimicrobials in this study. This finding highlights the importance of using these antimicrobials in controlling mycoplasma infections in chickens and turkeys.

Keywords: Mycoplasma species; broth microdilution qRT-PCR; toldin CRD; tylvalosin.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Growth inhibition rates of MG1 (A), MG2 (B), MG3 (C), and MS (D) grown into mycoplasma medium with various concentrations of tylvalosin at 2, 4, 7, and 14 d post incubation.
See this image and copyright information in PMC

References

    1. Ariela Boeder M., Tenfen A., Siebert D.A., Almeida C.L.B., Firmo C.R.M., Scharf D.R., Micke G.A., Siminionatto E.L., Cordova C.M.M., Guedes A. Anti-mycoplasma activity of Curcuma longa extracts and an isolated compound, the curcumin. Revista Fitos Rio de Janeiro. 2018;12:112–118.
    1. Behbahan N.G.G., Asasi K., Afsharifar A.R., Pourbakhsh S.A. Susceptibilities of Mycoplasma gallispeticum and Mycoplasma synoviae isolates to antimicrobial agents in vitro. Int. J. Poult. Sci. 2008;7:1058–1064.
    1. Brunner H., Laber G. In: The Mycoplasmas. Razin S., Barile M.F., editors. Vol. IV. Academic Press Inc.; Orlando, Fla: 1985. Chemotherapy of mycoplasma infections; pp. 403–450.
    1. Eissa S.I., Dardeer M.A., Hassan A.M., Abd Elaziz E.E., Al-Zeftawy N.M. In vitro and in vivo evaluation of some antimicrobials activity against Mycoplasma gallisepticum isolates from day old Chicks in Egypt. Zagazig Vet. J. 2009;37:82–91.
    1. Eissa S.I., Hassan A.M., Metwally A.M., Hashem Y.M., Abd El-Aziz E.E. Molecular characterization of Mycoplasma gallisepticum isolated from Chicken and Turkey. Giza. Vet. Med. J. 2011;59:183–195.

Uncited references

    1. Gerchman I., Lysnyansky I., Perk S., Levisohn S. In vitro susceptibilities to fluoroquinolones in current and archived Mycoplasma gallisepticum and Mycoplasma synoviae isolates from meat-type turkeys. Vet. Microbiol. 2008;131:266–276. - PubMed

MeSH terms

Substances