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. 2019 Dec;51(11):e13440.
doi: 10.1111/and.13440. Epub 2019 Oct 4.

Automation of human semen analysis using a novel artificial intelligence optical microscopic technology

Ashok Agarwal  1 , Ralf Henkel  1   2 , Chun-Chia Huang  3 , Maw-Sheng Lee  3   4
Affiliations

Automation of human semen analysis using a novel artificial intelligence optical microscopic technology

Ashok Agarwal et al. Andrologia. 2019 Dec.

Abstract

Current semen analysis still commonly depends on a manual microscopy method in clinical laboratories worldwide. However, some of the major disadvantages of this technique are that it is labour-intensive, subjective, laboratory-based and time-consuming. Although computer-assisted semen analysers (CASAs) have enabled partial automation of routine semen analysis, they lack wider acceptance due to their complicated operation. Therefore, the development of an accessible, rapid and standardised method for semen analysis is urgently needed. Here, we describe the development and clinical testing of a novel, automated, artificial intelligence optical microscopic (AIOM)-based technology, LensHooke™ X1 PRO (X1 PRO), designed for the quantitative measurement of sperm concentration, motility and seminal pH. We observed high degree of correlation in the results of concentration, progressive motility and progressively motile sperm concentration between the X1 PRO semen analyser and manual method using 135 clinical semen samples. In addition, the seminal pH results obtained by X1 PRO and manual methods were comparable (p = .12). In summary, our results showed that new X1 PRO semen analyser is a reliable diagnostic tool for routine semen analysis providing clinically acceptable results based on World Health Organization (WHO) 5th Edition guidelines.

Keywords: LensHook™ X1 PRO; automated semen analysis; computer-assisted sperm analysis; sperm concentration.

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References

REFERENCES

    1. Agarwal, A., Panner Selvam, M. K., Sharma, R., Master, K., Sharma, A., Gupta, S., & Henkel, R. (2018). Home sperm testing device versus laboratory sperm quality analyzer: Comparison of motile sperm concentration. Fertility and Sterility, 110(7), 1277-1284. https://doi.org/10.1016/j.fertnstert.2018.08.049
    1. Agarwal, A., & Sharma, R. K. (2007). Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer. Fertility and Sterility, 87(1), 156-162. https://doi.org/10.1016/j.fertnstert.2006.05.083
    1. Akashi, T., Mizuno, I., Okumura, A., & Fuse, H. (2005). Usefulness of sperm quality analyzer-V (SQA-V) for the assessment of sperm quality in infertile men. Archives of Andrology, 51(6), 437-442. https://doi.org/10.1080/014850190959081
    1. Amann, R. P., & Waberski, D. (2014). Computer-assisted sperm analysis (CASA): Capabilities and potential developments. Theriogenology, 81(1), 5-17.e11-13. https://doi.org/10.1016/j.theriogenology.2013.09.004
    1. Barratt, C. L., Tomlinson, M. J., & Cooke, I. D. (1993). Prognostic significance of computerized motility analysis for in vivo fertility. Fertility and Sterility, 60(3), 520-525.

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