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. 2019 Sep 20;37(9):690-693.
doi: 10.3760/cma.j.issn.1001-9391.2019.09.014.

[Evaluation of GeneXpert MTB/RIF and BACTEC-MGIT 960 for the detection of tuberculosis among pneumoconiosis-associated tuberculosis patients]

[Article in Chinese]
Y Jin  1 , H Q Wang  2 , J G Fan  3 , J Pang  4 , P Y Zhang  4 , T Li  2
Affiliations

[Evaluation of GeneXpert MTB/RIF and BACTEC-MGIT 960 for the detection of tuberculosis among pneumoconiosis-associated tuberculosis patients]

[Article in Chinese]
Y Jin et al. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. .

Abstract
in English, Chinese

Objective: To evaluate the performence of GeneXpert MTB/RIF and BACTEC-MGIT 960 on detecting Mycobacterium tuberculosis and rifampicin resistance for pneumoconiosis-associated tuberculosis patients. Methods: The recruited 133 suspected active pneumoconiosis-associated tuberculosis hospitalized cases, morning sputum samples were collected to do modified L-J culture, conventional proportion method drug susceptibility test, GeneXpert MTB/RIF and BACTEC-MGIT 960. Analyze the sensitivity and specificity of the 133 sputum from patients, the positive rates of patients with tuberculosis in GeneXpert MTB/RIF test, BACTEC-MGIT 960 and modified L-J culture were 37.59%, 34.59% and 30.08% respectively. There was no significant difference among the three tests respectively (P>0.05) . According to the modified L-J culture, the sensitivity of GeneXpert MTB/RIF and BACTEC-MGIT 960 in detecting tuberculosis were 92.5% and 95.0% respectively, and specificity in rifampicin resistance were 86.0% and 91.4% respectively. There was no significant difference between GeneXpert MTB/RIF and BACTEC-MGIT 960 (P>0.05) . According to conventional proportion method drug susceptibility test, the sensitivity of GeneXpert MTB/RIF and BACTEC-MGIT 960 in detecting rifampicin resistance were 90.0% and 100%, and specificity were 92.6% and 96.4%. There was no significant difference between GeneXpert MTB/RIF and BACTEC-MGIT 960 (P>0.05) . Conclusion: The GeneXpert MTB/RIF has good performence of detecting tuberculosis and rifampicin resistance. It has good application value among pneumoconiosis-associated tuberculosis patients.

目的： 探讨结核分枝杆菌/利福平耐药实时荧光定量核酸扩增检测体系（GeneXpert MTB/RIF）和结核分枝杆菌快速培养（BACTEC-MGIT 960）检测技术在尘肺结核诊断及耐药性测定中的应用。 方法： 选择2016年1月~12月期间湖南省胸科医院治疗的133例尘肺并发活动性肺结核病例，采集病人晨痰标本，分别进行改良罗氏培养法、GeneXpert MTB/RIF和BACTEC-MGIT 960检测。以改良罗氏法培养和比例法药敏试验结果为基准，评价GeneXpert MTB/RIF和BACTEC-MGIT 960检测结核分枝杆菌和利福平耐药效能。 结果： GeneXpert MTB/RIF、BACTEC-MGIT 960试验和改良罗氏法培养法检测结核分枝杆菌阳性率分别为37.59%、34.59%和30.08%，3种检测技术检出率无统计学差异（P>0.05）。以改良罗氏法培养为基准，GeneXpert MTB/RIF和BACTEC-MGIT 960检测结核分枝杆菌灵敏度分别为92.5%和95.0%，特异度分别为86.0%和91.4%，2种方法各观察指标间的差异均无统计学意义（P均>0.05）。以比例法药敏试验结果为基准，GeneXpert MTB/RIF和BACTEC-MGIT 960检测利福平耐药性灵敏度分别为90.0%和100.0%，特异度分别为92.6%和96.4%，两种方法各观察指标间的差异均无统计学意义（P均>0.05）。 结论： GeneXpert MTB/RIF检测在尘肺结核诊断中具有较高诊断效能，在实际工作中具有良好的应用价值。.

Keywords: Diagnosis; GeneXpert MTB/RIF; Mycobacterium tuberculosis culture (BACTEC-MGIT 960); Pneumoconiosis; Tuberculosis.

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