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Comment
. 2019 Nov 1;115(13):e133-e135.
doi: 10.1093/cvr/cvz265.

The epitranscriptome: tools to study, manipulate, and exploit RNA modifications

Anne Yaël Nossent  1   2   3   4
Affiliations
Comment

The epitranscriptome: tools to study, manipulate, and exploit RNA modifications

Anne Yaël Nossent. Cardiovasc Res. .
No abstract available

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Figures

Figure 1
Figure 1
Schematic overview of two novel epitranscriptome tools. (A) RNAs, including (pri- and pre-) microRNAs, as depicted here, can undergo posttranscriptional modifications. Both my group and others have shown that such modifications are induced by cellular stress, altering the function of the affected RNAs. (B) To detect modifications in small RNAs, total RNA is extracted from cells and the small RNA fraction is isolated. This fraction is further separated by size using high pressure (or high performance) liquid chromatography. 16–28 nucleotide long RNAs are hydrolyzed to single (modified) nucleotides, which are identified by mass spectrometry (Adapted from Lan et al.11). (C) The epitranscriptome can be manipulated specifically, potentially for future therapeutic applications. An ASO consisting of a target-site-specific domain and an ADAR-recruiting domain directs the deaminase enzyme ADAR to a target site in the transcriptome, leading to site-specific A-to-I editing of the target RNA (Adapted from Merkle et al.8). Image created by Vesna Krajina.
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See this image and copyright information in PMC

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References

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