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Comparative Study
. 2019 Dec;95(4):114880.
doi: 10.1016/j.diagmicrobio.2019.114880. Epub 2019 Aug 2.

FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens

Kerstin Locher  1 , Diane Roscoe  2 , Agatha Jassem  3 , Titus Wong  2 , Linda M N Hoang  3 , Marthe Charles  2 , Elizabeth Bryce  2 , Jennifer Grant  2 , Aleksandra Stefanovic  2
Affiliations
Comparative Study

FilmArray respiratory panel assay: An effective method for detecting viral and atypical bacterial pathogens in bronchoscopy specimens

Kerstin Locher et al. Diagn Microbiol Infect Dis. 2019 Dec.

Abstract

The BioFire FilmArray Respiratory Panel (FA RP) is a rapid multiplexed molecular assay approved for detection of viral and atypical bacterial pathogens in nasopharyngeal specimens. This study aimed to evaluate the performance of the BioFire FilmArray Respiratory Panel v1.7 on bronchoscopy specimens. We tested 133 bronchial specimens (87 archived and 46 prospectively collected) with the FA RP and compared the results to the Luminex NxTAG Respiratory Pathogen Panel (NxTAG RPP). After discordant analysis, 123 specimens gave concordant results using the FA RP and the NxTAG RPP for an overall agreement of 93.9% (kappa = 0.88 [95% CI 0.80-0.96]), a positive percent agreement of 93.7% (95% CI 83.7-97.7) and a negative percent agreement of 94.1% (95% CI 84.9-98.1). In conclusion, the BioFire FilmArray RP performed reliably to detect a broad range of respiratory pathogens in bronchoscopy specimens.

Keywords: Bronchoscopy; FilmArray respiratory panel; NxTag respiratory panel; Syndromic respiratory testing.

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