Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Sep 23;31(4):400-403.
doi: 10.16250/j.32.1374.2018269.

[Inhibition of Toxoplasma gondii excretory - secretory antigens on growth of murine Lewis lung carcinoma]

[Article in Chinese]
Jiao Yu-Meng  1 , Tao Zhi-Yong  1 , Cui Yu-Jian  1 , Liu Chun-Xiang  1 , Xia Hui  1 , Wang Xue-Mei  1 , Fang Qiang  1
Affiliations

[Inhibition of Toxoplasma gondii excretory - secretory antigens on growth of murine Lewis lung carcinoma]

[Article in Chinese]
Jiao Yu-Meng et al. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. .

Abstract
in English, Chinese

Objective: To investigate the effect of Toxoplasma gondii excretory-secretory antigens (ESA) on CD4+ CD25+ Foxp3+ T (Treg) cells in mice carrying Lewis lung carcinoma, and examine the inhibitory effect of T. gondii ESA on tumor growth.

Methods: C57BL/6 mice were randomly assigned into the PBS group (n = 14) and the Lewis group (n = 34). Mice in the Lewis group were subcutaneously injected with 2 × 105 Lewis lung carcinoma cells in the right axilla, while animals in the PBS group were injected with the same volume of sterile PBS. On day 7 post-injection (D7), mice in the PBS group were further divided into the PBS2 group and the PBS2 + ESA group, of 7 mice in each group, and mice in the Lewis group were further divided into the Lewis2 group and the Lewis2 + ESA group, of 17 mice in each group. Then, mice in the PBS2 + ESA group and the Lewis2 + ESA group were intraperitoneally injected with 100 μL of ESA. The mouse spleen coefficient was calculated in each group 7 days post-injection with ESA, and the changes of Treg cell counts and the long-term tumor growth were measured in tumor-bearing mice.

Results: The spleen coefficient was significantly greater in the PBS2 + ESA group and the Lewis2 + ESA group than in the PBS2 (0.66% ± 0.09% vs. 0.30% ± 0.02%, P < 0.05) and Lewis2 groups (0.69% ± 0.07% vs. 0.33% ± 0.03%, P < 0.05) 7 days post-treatment with ESA, respectively, and the percentage of splenic Treg cells in splenocytes was significantly lower in the PBS2 + ESA group and the Lewis2 + ESA group than in the PBS2 (1.28% ± 0.14% vs. 2.06% ± 0.07%, P < 0.05) and Lewis2 groups (1.58% ± 0.14% vs. 2.44% ± 0.23%, P < 0.05), respectively. T. gondii ESA treatment caused a delay in tumor growth, and the tumor size was significantly smaller in the Lewis2 + ESA group than in the Lewis2 group (P < 0.05).

Conclusions: T. gondii ESA may reduce the proportion of splenic Treg cells in splenocytes and inhibit tumor growth in mice carrying Lewis lung carcinoma.

[摘要] 目的 探讨弓形虫排泄分泌抗原 (ESA) 对 Lewis肺癌小鼠CD4+ CD25+ Foxp3+ T (Treg) 细胞亚群的影响, 观察弓 形虫ESA对肿瘤生长的抑制作用。方法 将C57BL/6小鼠随机分成PBS组 (14只) 和Lewis组 (34只)。Lewis组小鼠右腋 窝皮下接种Lewis肺癌细胞2 × 105个, PBS组注射等量无菌PBS。接种后第7 天 (D7), 将PBS组小鼠分成PBS2组、PBS2 + ESA组, 每组7只; 将Lewis组分成Lewis2组和Lewis2 + ESA组, 每组17只; PBS2 + ESA组及Lewis2 + ESA组小鼠腹腔注 射100 μL弓形虫ESA。ESA干预后7 d计算各组小鼠脾脏系数, 检测Treg细胞数量变化, 同时观察荷瘤鼠长期瘤体生长 情况。结果 ESA干预后7 d, PBS2 + ESA组 [(0.66 ± 0.09) %]和 Lewis2 + ESA组 [(0.69 ± 0.07) %]脾脏明显增大, 脾脏系 数与PBS2 组 [(0.30 ± 0.02) %]和Lewis2 组 [(0.33 ± 0.03) %]比较, 差异均有统计学意义 (P 均< 0.05)。PBS2 + ESA 组 [(1.28 ± 0.14) %]和Lewis2 + ESA组 [(1.58 ± 0.14) %]脾脏Treg细胞占脾细胞的比例均下降, 与PBS2组 [(2.06 ± 0.07) %]和 Lewis2组 [(2.44 ± 0.23) %]比较差异均有统计学意义 (P 均< 0.05)。ESA干预后, 瘤体生长延缓, 在实验终点时, Lewis2 + ESA组小鼠瘤体明显小于Lewis2组 (P < 0.05)。结论 ESA可下调荷瘤鼠脾脏Treg细胞占脾细胞的比例, 抑制瘤体生 长。.

Keywords: CD4+CD25+ Foxp3+T cell; Excretory-secretory antigen; Lung carcinoma; Mouse; Toxoplasma gondii.

PubMed Disclaimer

Substances