A novel label-free colorimetric aptasensor for sensitive determination of PSA biomarker using gold nanoparticles and a cationic polymer in human serum

Abstract

In this colorimetric assay for sensitive detection of prostate specific antigen (PSA) tumor marker, adsorbed non-thiolated poly-Adenine aptamer (polyA Apt) on the gold nanoparticles (AuNPs) surface was used. By incubating the AuNPs and the PSA specific aptamer prior to target addition, polyA Apt adsorbed on the gold nanoparticles and could bind the target while preventing non-specific interactions. Adsorbed polyA Apt on the AuNPs prevents aggregation of them by poly(diallyldimethylammoniumchloride) (PDDA). Upon the addition of PSA, it bind to the polyA Apt and induce the formation of a secondary structure. Therefore, interaction between polyA Apt and PDDA is repressed and PDDA induce the aggregation of the AuNPs. This analytical platform produces a remarkable optical signal in the absence and presence of PSA that accompanied by a color change from red to blue. This effect as a sensing strategy can be observed with naked eyes and quantified by colorimetry via measurement of the ratio of absorbances at 680 nm and 520 nm. Fabricated aptasensor for detection of PSA is linear in the concentration range of 0.1-100 ng/ml with 20 pg/ml as the limit of detection (S/N = 3). Because of the selectively recognized for PSA in the presence of other interfering substances, this proposed assay applied to real samples for the rapid screening of PSA.

Keywords: Colorimetric aptasensor; Gold nanoparticles; PSA biomarker; PolyA aptamer.