Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Editorial
. 2019 Sep 9:6:29.
doi: 10.21037/sci.2019.08.01. eCollection 2019.

SETD7 in cardiomyocyte differentiation and cardiac function

Tupa Basuroy  1 , Ivana L de la Serna  2
Affiliations
Editorial

SETD7 in cardiomyocyte differentiation and cardiac function

Tupa Basuroy et al. Stem Cell Investig. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Model showing stage-specific roles of SETD7 in CM differentiation. (A) At the MES stage, SETD7 binds the promoters and gene bodies of MES commitment genes (DKK1, MIXL1, and T). SETD7 is recruited to the promoters of MES commitment genes, presumably by interactions with as yet unidentified TFs, SETD7 then brings in the BRG1 component of the SWI/SNF complex and the histone acetyltransferase, p300, whose activities likely mobilize nucleosomes and acetylate histones respectively, thereby increasing chromatin accessibility. SETD7 also binds to H3K36me3 enriched regions of MES commitment gene bodies and is required for recruitment of elongating RNA polymerase (RNA PolII-ser2P); (B) in CP and CM stages, SETD7 interacts with cardiogenic TFs which cross-regulate each other’s expression and co-occupy cardiac specific promoters (MYH6, NKX2-5). One TF, NKX2-5 recruits SETD7 to a cardiac specific promoter (TNNT2). The function of SETD7 at cardiac promoters remains to be elucidated since an interaction between SETD7 and BRG1 was not detected at these later stages of CM differentiation. SETD7 also occupied the gene bodies of cardiac specific genes at H3K36me3 enriched regions in CPs and CMs and was required for recruitment of RNAPolII-ser2P. The co-occupancy of SETD7 with H3K36me3 was seen in terminally differentiated cells and adult heart tissue. SETD7 plays a role in cardiac function, primarily by regulating calcium handling. CM, cardiomyocyte; MES, mesoderm; TFs, transcription factors; SWI/SNF, switch/sucrose non-fermentable; CP, cardiac progenitor.
See this image and copyright information in PMC

Comment on

References

    1. Keenen B, de la Serna IL. Chromatin remodeling in embryonic stem cells: regulating the balance between pluripotency and differentiation. J Cell Physiol 2009;219:1-7. 10.1002/jcp.21654 - DOI - PubMed
    1. Saladi SV, de la Serna IL. ATP dependent chromatin remodeling enzymes in embryonic stem cells. Stem Cell Rev 2010;6:62-73. 10.1007/s12015-010-9120-y - DOI - PMC - PubMed
    1. Lee J, Shao NY, Paik DT, et al. SETD7 drives cardiac lineage commitment through stage-specific transcriptional activation. Cell Stem Cell 2018;22:428-44.e5. 10.1016/j.stem.2018.02.005 - DOI - PMC - PubMed
    1. Wang H, Cao R, Xia L, et al. Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Mol Cell 2001;8:1207-17. 10.1016/S1097-2765(01)00405-1 - DOI - PubMed
    1. Castaño J, Morera C, Sesé B, et al. SETD7 regulates the differentiation of human embryonic stem cells. PLoS One 2016;11:e0149502. 10.1371/journal.pone.0149502 - DOI - PMC - PubMed