Synergism between U.V. and thymidine treatments in the induction of cytogenetic damage in wild-type Friend erythroleukaemia cells

Abstract

The cytogenetic aberration-inducing effect of excess thymidine and U.V. light in Friend erythroleukaemia (clone 707) cells was investigated. Three doses of U.V. were utilised, namely 2.4, 4.8 and 7.2 J/m2. Thymidine, at 1 X 10(-5) M, was present for 48 h prior to U.V. treatment and for 15 h following it. Although no significant increase in metaphase aberrations was observed following thymidine treatment alone, relative to the spontaneous frequency, clone 707 exhibited increased sensitivity to U.V.-induced cytogenetic damage, when grown in the presence of 1 X 10(-5) M thymidine. The observed synergism between U.V. and thymidine treatments may be due to thymidine-induced nucleotide pool imbalance with consequent inaccuracies in DNA repair.