Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Nov;151(2):263-70.
doi: 10.1042/bj1510263.

Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland

S A Betts, R J Mayer

Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland

S A Betts et al. Biochem J. 1975 Nov.

Abstract

1. 6-Phosphogluconate dehydrogenase from rabbit mammary gland was purified to homogeneity by the criterion of polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The molecular weight of the subunit is 52 000. The enzyme was purified 150-fold with a final specific activity of 20 mumol of NADP+ reduced/min per mg of protein and overall yield of 3%. The molecular weight of the native enzyme is estimated to be 104 000 from gel-filtration studies. The final purification step was carried out by affinity chromatography with NADP+-Sepharose. 2. The Km values for 6-phosphogluconate and NADP+ are approx. 54 muM and 23 muM respectively. 3. Citrate and pyrophosphate are competitive inhibitors of the enzyme with respect to both 6-phosphogluconate and NADP+. 4. MgCl2 affects the apparent Km for NADP+ at saturating concentrations of 6-phosphogluconate.

PubMed Disclaimer

References

    1. Biochem J. 1953 Oct;55(3):400-8 - PubMed
    1. J Exp Med. 1959 Dec 1;110:899-919 - PubMed
    1. J Biol Chem. 1961 Nov;236:2975-80 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Biochim Biophys Acta. 1973 Apr 28;304(2):231-5 - PubMed