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Review
. 2020 Jan;43(1):313-336.
doi: 10.1002/jssc.201900804. Epub 2019 Nov 6.

Enrichments of post-translational modifications in proteomic studies

Luisa Pieroni  1 , Federica Iavarone  2   3 , Alessandra Olianas  4 , Viviana Greco  2   3 , Claudia Desiderio  5 , Claudia Martelli  6 , Barbara Manconi  4 , Maria Teresa Sanna  4 , Irene Messana  5 , Massimo Castagnola  1 , Tiziana Cabras  4
Affiliations
Review

Enrichments of post-translational modifications in proteomic studies

Luisa Pieroni et al. J Sep Sci. 2020 Jan.

Abstract

More than 300 different protein post-translational modifications are currently known, but only a few have been extensively investigated because modified proteoforms are commonly present in sub-stoichiometry amount. For this reason, improvement of specific enrichment techniques is particularly useful for the proteomic characterization of post-translationally modified proteins. Enrichment proteomic strategies could help the researcher in the challenging issue to decipher the complex molecular cross-talk existing between the different factors influencing the cellular pathways. In this review the state of art of the platforms applied for the enrichment of specific and most common post-translational modifications, such as glycosylation and glycation, phosphorylation, sulfation, redox modifications (i.e. sulfydration and nitrosylation), methylation, acetylation, and ubiquitinylation, are described. Enrichments strategies applied to characterize less studied post-translational modifications are also briefly discussed.

Keywords: enrichment; post-translational modifications; proteomics.

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