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. 2019 Oct 3:12:3113-3124.
doi: 10.2147/IDR.S206691. eCollection 2019.

Characterization of phenotypic and genotypic traits of carbapenem-resistant Acinetobacter baumannii clinical isolates recovered from a tertiary care hospital in Taif, Saudi Arabia

Mohamed F El-Badawy  1   2 , Sayed F Abdelwahab  1   3 , Saleh A Alghamdi  4 , Mohamed M Shohayeb  1   5
Affiliations

Characterization of phenotypic and genotypic traits of carbapenem-resistant Acinetobacter baumannii clinical isolates recovered from a tertiary care hospital in Taif, Saudi Arabia

Mohamed F El-Badawy et al. Infect Drug Resist. .

Abstract

Background and objective: Acinetobacter baumannii (A. baumannii) is a common nosocomial pathogen, which developed multi-drug-resistance to different classes of antibiotics including carbapenems. This study examined ten common carbapenemase genes among 32 carbapenem-resistant A. baumannii clinical isolates recovered from Taif, Saudi Arabia.

Methods: Isolates were phenotypically identified to the genus level by Vitek®2 and API 20NE®. The species level was confirmed by the amplification of bla OXA-51. The susceptibility for 21 different antibiotics was performed by Vitek 2 and modified Kirby-Bauer method. Isolates were genetically screened for 10 carbapenemases. Phylogenetic relatedness between isolates was determined by ERIC-PCR.

Results: Genotypically identified A. baumannii represented 100% of the total phenotypically identified Acinetobacter spp. All the carbapenem-resistant isolates were sensitive to polymyxin B and colistin. Among the other antibiotics, ampicillin/sulbactam and tigecycline were the most effective agents. 90.8% of the isolates were resistant to all ten investigated β-lactams. bla OXA-51, bla IPM, bla NDM and bla OXA-23 were detected in 100%, 87.5%, 62.5% and 59.4% of isolates, respectively. Also, bla VIM and bla OXA-40 were less prevalent and were detected in 9.3% and 3.1% of the isolates, respectively. In addition, bla KPC, bla OXA-48, bla OXA-58, bla OXA-181 were not detected in any isolate. The A. baumannii isolates were categorised into ten genotypes on the basis of the detected carbapenemase genes and ERIC-PCR revealed a remarkable clonal diversity among these isolates.

Conclusion: Class A and class D carbapenemase genes were the most commonly detected among carbapenem resistant A. baumannii (CRAB) clinical isolates.

Keywords: A. baumannii; ERIC-PCR; blaOXA-51; carbapenemases; carbapenems.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
DNA fingerprint pattern generated by ERIC-PCR for Acinetobacter clinical isolates. L, 100bp DNA ladder; lanes 1, 2, 3, 27,19, 26 and 32 represent the code No. of the isolates.
Figure 2
Figure 2
Clonal relationship between A. baumannii clinical isolates generated by UPGMA dendrogram.
See this image and copyright information in PMC

References

    1. Rahman A, Iza N, Ismail S, et al. Acinetobacter spp. infections in Malaysia: a review of antimicrobial resistance trends, mechanisms and epidemiology. Front Microbiol. 2017;8:2479. doi:10.3389/fmicb.2017.02479 - DOI - PMC - PubMed
    1. Kim B, Bae W, Kim K, Lee H, Yoon J. Nosocomial infection due to Acinetobacter baumannii in korean ICUs a multicenter study. Crit Care Med. 2018;46(1):343. doi:10.1097/01.ccm.0000528728.54507.7e - DOI - PubMed
    1. Almaghrabi MK, Joseph MR, Assiry MM, Hamid ME. Multidrug-resistant Acinetobacter baumannii: an emerging health threat in Aseer Region, Kingdom of Saudi Arabia. Can J Infect Dis Med Microbiol. 2018;2018. doi:10.1155/2018/9182747. - DOI - PMC - PubMed
    1. Majid Bouzari D, Abedini F, Yadegari S. Identification of genomic species of Acinetobacter isolated from burns of ICU patients. Arch Iran Med. 2015;18(10):638–642. - PubMed
    1. Al Atrouni A, Joly-Guillou M-L, Hamze M, Kempf M. Reservoirs of non-baumannii Acinetobacter species. Front Microbiol. 2016;7:49. - PMC - PubMed