Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Sep 17:2019:3865121.
doi: 10.1155/2019/3865121. eCollection 2019.

Network Pharmacology Reveals the Molecular Mechanism of Cuyuxunxi Prescription in Promoting Wound Healing in Patients with Anal Fistula

Yin Qu  1 , Zhijun Zhang  1 , Yafeng Lu  1 , De Zheng  1 , Yang Wei  1
Affiliations

Network Pharmacology Reveals the Molecular Mechanism of Cuyuxunxi Prescription in Promoting Wound Healing in Patients with Anal Fistula

Yin Qu et al. Evid Based Complement Alternat Med. .

Abstract

Background: The healing process of the surgical wound of anal fistulotomy is much slower because of the presence of stool within the wound. Cuyuxunxi (CYXX) prescription is a Chinese herbal fumigant that is being used to wash surgical wound after anal fistulotomy. This study aimed at investigating the molecular mechanism of CYXX prescription using a network pharmacology-based strategy.

Materials and methods: The active compounds in each herbal medicine were retrieved from the traditional Chinese medicine systems pharmacology (TCMSP) database and in Traditional Chinese Medicine Integrated Database (TCMID) analysis platform based on the criteria of oral bioavailability ≥40% and drug-likeness ≥0.2. The disease-related target genes were extracted from the Comparative Toxicogenomics Database. Protein-protein interaction network was built for the overlapped genes as well as functional enrichment analysis. Finally, an ingredient-target genes-pathway network was built by integrating all information.

Results: A total of 375 chemical ingredients of the 5 main herbal medicines in CYXX prescription were retrieved from TCMSP database and TCMID. Among the 375 chemical ingredients, 59 were active compounds. Besides, 325 target genes for 16 active compounds in 3 herbal medicines were obtained. Functional enrichment analysis revealed that these overlapped genes were significantly related with immune response, biosynthesis of antibiotics, and complement and coagulation cascades. A comprehensive network which contains 133 nodes (8 disease nodes, 3 drug nodes, 8 ingredients, 103 target gene nodes, 7 GO nodes, and 4 pathway nodes) was built.

Conclusion: The network built in this study might aid in understanding the action mechanism of CYXX prescription at molecular level to pathway level.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
The herb-active compounds network. A compound node and an herb are linked if the compound is in the herb.
Figure 2
Figure 2
The ingredient-target genes-pathway network.
Figure 3
Figure 3
Subnetwork analysis.
See this image and copyright information in PMC

References

    1. Feng S. T., Huang M., Dong Z., et al. MRI T2-weighted imaging and fat-suppressed T2-weighted imaging image fusion technology improves image discriminability for the evaluation of anal fistulas. Korean Journal of Radiology. 2019;20(3):429–437. doi: 10.3348/kjr.2018.0260. - DOI - PMC - PubMed
    1. Sainio P. Fistula-in-ano in a defined population. Incidence and epidemiological aspects. Annales Chirurgiae et Gynaecologiae. 1984;73(4):219–224. - PubMed
    1. Zanotti C., Martinez-Puente C., Pascual I., Pascual M., Herreros D., Garcia-Olmo D. An assessment of the incidence of fistula-in-ano in four countries of the European Union. International Journal of Colorectal Disease. 2007;22(12):1459–1462. doi: 10.1007/s00384-007-0334-7. - DOI - PubMed
    1. Malik A. I., Nelson R. L. Surgical management of anal fistulae: a systematic review. Colorectal Disease. 2008;10(5):420–430. doi: 10.1111/j.1463-1318.2008.01483.x. - DOI - PubMed
    1. Dudukgian H., Abcarian H. Why do we have so much trouble treating anal fistula? World Journal of Gastroenterology. 2011;17(28):3292–3296. doi: 10.3748/wjg.v17.i28.3292. - DOI - PMC - PubMed

LinkOut - more resources