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. 2019 Oct 16:7:e7859.
doi: 10.7717/peerj.7859. eCollection 2019.

Full genome sequence analysis of a 1-7-4-like PRRSV strain in Fujian Province, China

Jiankui Liu  1   2 , Chunhua Wei  1   2 , Zhifeng Lin  3 , Wei Xia  1   2 , Ying Ma  1   2 , Ailing Dai  1   2 , Xiaoyan Yang  1   2
Affiliations

Full genome sequence analysis of a 1-7-4-like PRRSV strain in Fujian Province, China

Jiankui Liu et al. PeerJ. .

Abstract

PRRS virus (PRRSV) has undergone rapid evolution and resulted in immense economic losses worldwide. In the present study, a PRRSV strain named FJ0908 causing high abortion rate (25%) and mortality (40%) was detected in a swine herd in China. To determine if a new PRRSV genotype had emerged, we characterized the genetic characteristics of FJ0908. Phylogenetic analysis indicated that FJ0908 was related to 1-7-4-like strains circulating in the United States since 2014. Furthermore, the ORF5 sequence restriction fragment length polymorphism (RFLP) pattern of FJ0908 was 1-7-4. Additionally, FJ0908 had a 100 aa deletion (aa329-428) within nsp2, as compared to VR-2332, and the deletion pattern was consistent with most of 1-7-4 PRRSVs. Collectively, the data of this study contribute to the understanding of 1-7-4-like PRRSV molecular epidemiology in China.

Keywords: 1-7-4; Genome sequence; Molecular characteristic; PRRSV.

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Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1. Phylogenetic tree based on the ORF5 genes (A) and full length (B) of the FJ0908 and reference viruses.
Reliability of the tree was assessed by bootstrap analysis of 1,000 replications. Our representative isolate FJ0908 were marked with the red triangle (▴). Lineage 1 PRRSVs are divided into nine sublineages.
Figure 2
Figure 2. Characterization of the supported recombinant events between FJL0908 and representative PRRSV lineages.
(A) Similarity plot and bootscan analyses of FJ0908 by SimPlot. The y-axis indicates the percentage similarity between the parental sequences and the query sequence. Phylogenetic trees based on major parental regions (nt 1-760 and nt 1301-15534) (B) and the minor parental region (nt 760–1300) (C). The major parental group (1-7-4 viruses, reference strain IA/2014/NADC34) is shown in red, while the minor parental groups (reference strain ISU30) are shown in blue, respectively.
See this image and copyright information in PMC

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