Marimonas lutisalis sp. nov., isolated from a tidal mudflat and emended description of the genus Marimonas

Abstract

A Gram-reaction-negative bacterial strain, designated GH1-19^T, was isolated from a tidal mudflat sample collected in Gangwha Island, Republic of Korea. Cells of the novel micro-organism were strictly aerobic, non-sporulating, motile and rod-shaped. Growth occurred at 10-40 °C (optimum, 30 °C), pH 6-9 (pH 8) and in the presence of 1-9 % NaCl (3 %). Comparative analysis of complete or nearly complete 16S rRNA gene sequences exhibited that strain GH1-19^T formed a distinct cluster between Marimonas arenosa CAU 1311^T (97.42 % sequence similarity) and Aquicoccus porphyridii L1 8-17^T (97.35 %). Similarity levels of 16S rRNA gene sequences between the novel strain and other members of the family Rhodobacteraceae were below 96.6 %. The isoprenoid quinone was Q-10. The major fatty acids were C_{18 : 1}ω7c, C_{16 : 0}, summed feature 3 (C_{16 : 0} ω7c and/or C_{16 : 0} ω6c) and C_{12 : 0} 3-OH. The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid. The G+C content of the DNA was 63.2 mol% (draft genome). DNA-DNA relatedness value between the novel strain and the type strain of Marimonas arenosa was 12.7±9.0 %. On the basis of data from phenotypic, chemotaxonomic and DNA-DNA hybridization studies together with phylogenetic analyses, strain GH1-19^T (=KCTC 62376^T=DSM 106292^T) represents a novel species of the genus Marimonas, for which the name Marimonas lutisalis sp. nov. is proposed, with the emended description of the genus Marimonas.

Keywords: 16S rRNA gene; Aquicoccus; Marimonas lutisalis; genome; tidal mudflat.