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. 2020:2078:51-69.
doi: 10.1007/978-1-4939-9929-3_4.

Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies

Pragya Adhikari  1 , Neelie Zacharias  1 , Rachana Ohri  1 , Jack Sadowsky  2
Affiliations

Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies

Pragya Adhikari et al. Methods Mol Biol. 2020.

Abstract

Antibodies bearing engineered cysteine residues (termed THIOMAB™ antibodies) enable the site-selective attachment of a drug, label or other payload for specific delivery to certain tissues (e.g., tumors). This Chapter describes detailed methods we have developed and optimized for the conjugation, purification and analysis of THIOMAB™ antibody drug conjugates (TDCs).

Keywords: ADC; Disulfide; Engineered cysteine; Maleimide; Site-specific conjugation; THIOMAB.

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