Correction: Synthesis of IL-6 by Hepatocytes Is a Normal Response to Common Hepatic Stimuli

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0096053.].

Fig 3. IL-6 synthesis and NFκB signaling in hepatocytes after LPS injection.

(A) FISH for IL-6 mRNAs in serum-free rat hepatocyte cultures, 15 min after media change with 1 μg LPS/ml or diluent (control). For these photographs, as a baseline level of IL-6 mRNA was known to be present (see 1D), gating was adjusted with the diluent-treated sample serving as the baseline. (B, C) Rat livers were injected with 100 μg/kg LPS or saline (control) and harvested at 4 h post treatment. In B, samples were simultaneously stained for albumin protein (green) and IL-6 mRNA (red). Co-localization (merge) appears as yellow. A presumptive inflammatory cell (expressing IL-6 mRNA but albumin-negative) is indicated by an arrow and featured in magnified inserts. Mock hybridizations (not shown) were used as the immunofluorescent gating control. C shows standard immunohistochemical staining using an antibody against IL-6. (D, E) Representative immunohistochemistry depicting nuclear p65 (D), or p50 (E) with and without LPS treatment. Arrows show nuclei stained with p65 or p50 (brown), arrowheads indicate unstained nuclei (colorless). Simultaneously stained tissue section from liver of ILK-null mice [40] shows positive nuclear localization staining for p50 (left panel of D). Dotted boxes are featured in magnified inserts. Scale bars, 20 μm in all images except for D (40 μm).