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. 2019 Jul 11:6:84.
doi: 10.1038/s41438-019-0166-y. eCollection 2019.

Overexpression of CmMYB15 provides chrysanthemum resistance to aphids by regulating the biosynthesis of lignin

Cong An #  1 , Liping Sheng #  1 , Xinping Du  1 , Yinjie Wang  1 , Yi Zhang  1 , Aiping Song  1 , Jiafu Jiang  1 , Zhiyong Guan  1 , Weimin Fang  1 , Fadi Chen  1 , Sumei Chen  1
Affiliations

Overexpression of CmMYB15 provides chrysanthemum resistance to aphids by regulating the biosynthesis of lignin

Cong An et al. Hortic Res. .

Abstract

MYB transcription factors are widely involved in the development of and physiological processes in plants. Here, we isolated the chrysanthemum R2R3-MYB family transcription factor CmMYB15, a homologous gene of AtMYB15. It was demonstrated that CmMYB15 expression was induced by aphids and that CmMYB15 could bind to AC elements, which usually exist in the promoter of lignin biosynthesis genes. Overexpression of CmMYB15 in chrysanthemum enhanced the resistance of aphids. Additionally, the content of lignin and the expression of several lignin biosynthesis genes increased. In summary, the results indicate that CmMYB15 regulates lignin biosynthesis genes that enhance the resistance of chrysanthemum to aphids.

Keywords: Biotic; Transcriptional regulatory elements; Transgenic plants.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. Putative peptide sequences of CmMYB15 and other MYB proteins.
a Alignment of the putative amino acid sequence of CmMYB15 (boxed) with that of related MYB proteins that feature an R2MYB domain or an R3MYB domain (underlined), b Phylogenetic analysis of the relationships between CmMYB15 and other MYB proteins from Arabidopsis. The sequence details are as follows: AtMYB15 (AT3G23250.1), LmMYB1 (ADN96004.1), MnMYB4 (XP_010095860.1), TaMYB33 (AEO21928.1), TaMYB73 (XP_020169006.1), GmMYB29A2 (BAA81732.1), ATMYB61 (AT1G09540.1), AtMYB50 (AT1G57560.1), AtMYB55 (AT4G01680.1), ATMYB86 (AT5G26660.1), AtMYB103 (AT1G63910.1), ATMYB67 (AT3G12720.1), ATMYB26 (AT3G13890.1), AtMYB83 (AT3G08500.1), ATMYB46 (AT5G12870.1), AtMYB18 (AT4G25560.1), AtMYB45 (AT3G48920.1), ATMYB95 (AT1G74430.1), AtMYB60 (AT1G08810.1), ATMYB13 (AT1G06180.1), ATMYB58 (AT1G16490.1), ATMYB63 (AT1G79180.1), AtMYB24 (AT5G40350.1), ATMYB21 (AT3G27810.1), AtMYB27 (AT3G53200.1), ATMYB59 (AT5G59780.3), and ATMYB48 (AT3G46130.1)
Fig. 2
Fig. 2
Subcellular localization of CmMYB15. Bar  = 100  μm
Fig. 3
Fig. 3. Transcriptional activation activity analyses of CmMYB15 and the DNA-binding assay of AC elements with CmMYB15.
a The transcriptional activity analysis of CmMYB15 via a yeast assay system, in which pCL1 is a positive control and pGBKT7 is a negative control. b Yeast one-hybrid (Y1H) assay of CmMYB15 with the three kinds of AC elements: AC-I, AC-II, and AC-III, as well as their mutated form mAC
Fig. 4
Fig. 4. Expression of CmMYB15 in different organs of chrysanthemum plants and under aphid and puncture treatment, as revealed by quantitative real-time PCR.
a Expression of CmMYB15 in the roots, stems and leaves of wild-type chrysanthemum, b Expression of CmMYB15 in response to aphid treatment and puncture treatment in the third fully expanded leaf counted from the apex. The bars indicate the standard errors
Fig. 5
Fig. 5. The aphid resistance of wild-type chrysanthemum and CmMYB15-overexpressing chrysanthemum.
a Identification of CmMYB15-overexpressing chrysanthemum plants by PCR, in which the plasmid pMDC43-2 × 35 S::CmMYB15 acted as a positive control and wild-type (WT) acted as a negative control. -ox1, -ox2, -ox3 and -ox4 represent transgenic plants overexpressing CmMYB15, b transcription level of CmMYB15 in wild-type and CmMYB15-overexpressing chrysanthemum plants, c differential proliferation of aphids between CmMYB15-overexpressing and nontransgenic plants, d average number of aphids on CmMYB15-overexpressing and wild-type plants measured at 3–21 days after aphid infestation
Fig. 6
Fig. 6. The lignin content and expression levels of lignin biosynthesis genes in CmMYB15-overexpressing plants.
a The lignin content of wild-type and CmMYB15-overexpressing plants, b expression levels of eight lignin biosynthesis genes in wild-type and CmMYB15-overexpressing plants. The bars indicate the standard errors
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