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. 2019 Sep 16;10(10):5162-5181.
doi: 10.1364/BOE.10.005162. eCollection 2019 Oct 1.

Polarization-sensitive optical coherence elastography

Arata Miyazawa  1 , Shuichi Makita  1 , En Li  1 , Kohei Yamazaki  2 , Masaki Kobayashi  2 , Shingo Sakai  3 , Yoshiaki Yasuno  1
Affiliations

Polarization-sensitive optical coherence elastography

Arata Miyazawa et al. Biomed Opt Express. .

Abstract

Polarization-sensitive optical coherence elastography (PS-OCE) is developed for improved tissue discrimination. It integrates Jones matrix-based PS-optical coherence tomography (PS-OCT) with compression OCE. The method simultaneously measures the OCT intensity, attenuation coefficient, birefringence, and microstructural deformation (MSD) induced by tissue compression. Ex vivo porcine aorta and esophagus tissues were investigated by PS-OCE and histological imaging. The tissue properties measured by PS-OCE are shown as cross-sectional images and a three-dimensional (3-D) depth-trajectory plot. In this trajectory plot, the average attenuation coefficient, birefringence, and MSD were computed at each depth, and the trajectory in the depth direction was plotted in a 3-D feature space of these three properties. The tissue boundaries in a histological image corresponded with the depth-trajectory inflection points. Histogram analysis and t-distributed stochastic neighbour embedding (t-SNE) visualization of the three tissue properties indicated that the PS-OCE measurements provide sufficient information to discriminate porcine esophagus tissues.

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Conflict of interest statement

AM, EL: Kao Corp. (F), Yokogawa Electric Corp. (F), Topcon (F), Tomey Corp (F), Nidek (F). SM, YY: Kao Corp. (F), Yokogawa Electric Corp. (F), Topcon (F), Tomey Corp (F, P), Nidek (F). KY, MK, SS: (E) Kao Corp.

Figures

Fig. 1.
Fig. 1.
Schematic of the system. C: coupler; FBG: fiber Bragg grating; PC: polarization controller; LP: linear polarizer; PBS: polarizing beam splitter; RAP: right angle prism; NPBS: non-polarization beam splitter; BPD: balanced photodetetor; LPF: low-pass filter; HPF: high-pass filter.
Fig. 2.
Fig. 2.
(a) H&E and (b) EVG histologies of the aorta. (c) OCT intensity, (d) attenuatin coefficient, (e) birefringence, and (f) MSD images of the aorta. The scale bars represent 0.5 mm × 0.5 mm. Arrow heads indicate the interface between the tunica media and the tunica externa. The surface of the glass plate appears as a hyperreflective line above the tissue surface in (c), (d), (e) and (f).
Fig. 3.
Fig. 3.
High-definition image of EVG histology of aorta. (b), (c), and (d) show enlarged images of the yellow windows in (a). The scale bars represent 0.5 mm × 0.5 mm.
Fig. 4.
Fig. 4.
(a), (b) Depth-trajectory plots for the aorta from various angles (see also Visualization 1).
Fig. 5.
Fig. 5.
(a) H&E and (b) EVG histologies of esophagus. (c) OCT intensity, (d) attenuation coefficient, (e) birefringence, and (f) MSD images of esophagus. The scale bars represent 0.5 mm × 0.5 mm. MucEp: mucosal epithelium; LaPr: lamina propria; MusMuc: muscularis mucosa; SubMuc: submucosa; MusEx: muscularis externa. The surface of the glass plate appears as a hyperreflective line above the tissue surface in (c), (d), (e) and (f).
Fig. 6.
Fig. 6.
(a), (b) Depth-trajectory plots of esophagus from various angles (see also Visualization 2).
Fig. 7.
Fig. 7.
Distributions of (a) five ROIs and (b) three ROIs of porcine esophagus when visualized using t-SNE.
See this image and copyright information in PMC

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