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Case Reports
. 2019 Dec;48(4):624-629.
doi: 10.1111/vcp.12792. Epub 2019 Oct 24.

Abnormal Sysmex XT-2000iV DIFF scattergram in a cat with a prominent mastocytemia

Typhaine Lavabre  1 , Adeline Betting  2 , Nathalie Bourgès-Abella  1 , Catherine Layssol-Lamour  1 , Catherine Trumel  1
Affiliations
Case Reports

Abnormal Sysmex XT-2000iV DIFF scattergram in a cat with a prominent mastocytemia

Typhaine Lavabre et al. Vet Clin Pathol. 2019 Dec.

Abstract

A 2-year-old neutered male domestic shorthair cat was presented to the emergency service of the National Veterinary School of Toulouse (France) for acute vomiting and diarrhea with lethargy, inappetence, and adypsia for the past 48 hours. Complete blood counts were performed with the ProCyte DX at the emergency department and with the Sysmex XT-2000iV at the laboratory 2 weeks later. The scattergrams from the two analyzers revealed similar unusual and abnormal dot plots. The Sysmex XT-2000iV DIFF scattergram also showed no clear separation between different leukocyte populations. The eosinophil cluster was in an abnormal location compared with that of the "typical" location in a normal cat. A blood smear evaluation revealed the presence of numerous mast cells. Thus, we hypothesized that the Sysmex XT-2000iV had detected the mast cell population, and this led to errors in the differential counts. To explore this hypothesis, we manually gated on the DIFF scattergram and performed a manual differential on the blood smear. With this new gating strategy, the Sysmex XT-2000iV and manual differentials were similar. Thus, in the case of systemic mastocytosis, mast cells can be located between the lymphocyte, monocyte, and eosinophil clusters on scattergrams.

Keywords: Differential leukocyte count; feline blood; laser counter; lysis-resistant granulocytes; mastocytosis.

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References

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