Localized cell death caused by mutations in a Drosophila gene coding for a transforming growth factor-beta homolog

Abstract

Recessive mutations (dppdisk) in one region of the decapentaplegic (dpp) gene of Drosophila, which codes for a transforming growth factor-beta homolog, cause loss of distal parts from adult appendages. Different dppdisk alleles cause effects of different severity, the milder alleles removing distal parts and the more severe alleles removing progressively more proximal structures. In the wing disc derivatives, the most extreme dppdisk genotype removes the entire wing and leaves only a thorax fragment. We show that structures are lost in these mutants as a result of massive apoptotic cell death in the corresponding regions of the imaginal discs during the mid-third larval instar. The remaining disc fragments do not regenerate when cultured alone in the growth-permissive environment of the adult abdomen, but they can be made to regenerate by coculturing them with appropriate fragments of wild-type wing discs. This nonautonomous development is interpreted as showing that a product of dpp+, presumably the TGF-beta homolog, is secreted by the normal cells and can rescue the mutant cells in the mixed tissue.