Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Oct 24;12(1):688.
doi: 10.1186/s13104-019-4732-z.

Anti-Toxoplasma activity of Sorghum bicolor-derived lipophilic fractions

Daniel A Abugri  1 , Jesse M Jaynes  2 , William H Witola  3
Affiliations

Anti-Toxoplasma activity of Sorghum bicolor-derived lipophilic fractions

Daniel A Abugri et al. BMC Res Notes. .

Abstract

Objective: Toxoplasma gondii, an intracellular zoonotic parasite, infects approximately a third of the world population. Current drugs for treatment of T. gondii infection have been challenged with ineffectiveness and adverse side effects. This necessitates development of new anti-Toxoplasma drugs. Sorghum bicolor [Moench] leaf extract has been used in African traditional medicine for the management of anemia and treatment of infectious diseases. We tested the in vitro anti-Toxoplasma inhibitory activity of S. bicolor's oil-like crude extracts and fractions against T. gondii and determined their cytotoxic effects on human host cells.

Results: Significant inhibitory activities against the growth of T. gondii tachyzoites were observed for the crude extract (IC50 = 3.65 µg/mL), the hexane-methanol fraction (IC50 = 2.74 µg/mL), and the hexane fraction (IC50 = 3.55 µg/mL) after 48 h of culture. The minimum cytotoxicity concentrations against HFF were 34.41, 16.92 and 7.23 µg/mL for crude extract, hexane-methanol and hexane fractions, respectively. The crude extract and fractions showed high antiparasitic effects with low cytotoxic effects. Further studies to determine synergistic activities and modes of action would provide impetus for the development of new toxoplasmosis drugs or nutraceuticals.

Keywords: Anti-Toxoplasma activity; Chemical composition; Oil-like extracts/fractions; Sorghum bicolor.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
a Cytotoxic effects of lipophilic S. bicolor leaves crude extract on Human foreskin fibroblasts cells. b Cytotoxic effects of the hexane fraction from the lipophilic Sorghum bicolor extract on human foreskin fibroblast cells. cCytotoxic effects of the hexane-methanol (10:1) fraction from the lipophilic Sorghum bicolor extract on human foreskin fibroblast cells
See this image and copyright information in PMC

References

    1. Dubey JP, Beattie CP. Toxoplasmosis of animals and man. Boca Raton: CRC Press, Inc.; 1988. Toxoplasmosis in man (Homo sapiens) pp. 1–336.
    1. Zhang M, Yang Z, Wang S, Tao LF, Xu L, Song RX, Li X. Detection of Toxoplasma gondii in shellfish and fish in parts of China. Vet Parasitol. 2014;200:85–89. doi: 10.1016/j.vetpar.2013.10.022. - DOI - PubMed
    1. Flegr J, Prandota J, Soviekova M, Israili ZH. Toxoplasmsosis—a global threat. Correlation of latent toxoplasmsosis with specific disease burden in a set of 88 countries. PLoS ONE. 2014;9:e90203. doi: 10.1371/journal.pone.0090203. - DOI - PMC - PubMed
    1. Dubey JP. Toxoplasmosis—a waterborne zoonosis. Vet Parasitol. 2006;126:57–72. doi: 10.1016/j.vetpar.2004.09.005. - DOI - PubMed
    1. Tenter AM, Heckeroth AR, Weiss LM. Toxoplasma gondii: from animals to humans. Int J Parasitol. 2000;30:1217–1258. doi: 10.1016/S0020-7519(00)00124-7. - DOI - PMC - PubMed

MeSH terms

Grants and funding

LinkOut - more resources