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. 2019 Oct 24;24(21):3836.
doi: 10.3390/molecules24213836.

Analysis of Nitraria Tangutourum Bobr-Derived Fatty Acids with HPLC-FLD-Coupled Online Mass Spectrometry

Na Hu  1   2   3 , Jian Ouyang  4 , Qi Dong  5   6 , Honglun Wang  7   8   9
Affiliations

Analysis of Nitraria Tangutourum Bobr-Derived Fatty Acids with HPLC-FLD-Coupled Online Mass Spectrometry

Na Hu et al. Molecules. .

Abstract

Fatty acids (FAs) are basic components in plants. The pharmacological significance of FAs has attracted attentions of nutritionists and pharmaceutists. Sensitive and accurate detection of FAs is of great importance. In the present study, a pre-column derivatization and online mass spectrometry-based qualitative and quantitative analysis of FAs was developed. Nineteen main FAs were derivatized by 2-(7-methyl-1H-pyrazolo-[3,4-b]quinoline-1-yl)ethyl-4-methyl benzenesulfonate (NMP) and separated on reversed-phase Hypersil BDS C8 column with gradient elution. All FAs showed excellent linear responses with correlation coefficients more than 0.9996. The method obtained LOQs between 0.93 ng/mL and 5.64 ng/mL. FA derivatives were identified by both retention time and protonated molecular ion corresponding to m/z [M + H]+. A comparative study based on FA contents in peel and pulp, seeds and leaves of Nitraria tangutourum Bobr (NTB) from different geographical origins was performed with the established method. Results indicated that NTB were rich in FAs, and the types and contents of FAs varied among tissues. On the other hand, the same tissue of NTB from different geographical areas differed in the content, but not in type, of FAs.

Keywords: HPLC-FLD; Nitraria tangutourum Bobr; different geographical origins; fatty acids; online mass spectrometry.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Optimization of derivatization conditions ((A): Co-solvent; (B): catalysts; (C): molar ratio; (D): temperature; (E): time).
Figure 2
Figure 2
MS spectra of the representative oleic acid derivative and fragmentation pattern of the protonated molecular ion. (A) molecular ion peak of the oleic acid derivative. (B) fragmentation pattern of protonated molecular ion
Figure 3
Figure 3
Representative chromatograms for standards (A), fatty acid derivatives in seeds of Nitraria tangutorum Bobr. (B), fatty acid derivatives in peel and pulp of Nitraria tangutorum Bobr. (C) and fatty acid derivatives in leaves of Nitraria tangutorum Bobr. (D).
Figure 4
Figure 4
Derivatization reaction process of the derivatized reagent NMP and fatty acid.
See this image and copyright information in PMC

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