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Review
. 2020 Jan;43(1):258-270.
doi: 10.1002/jssc.201900758. Epub 2019 Nov 12.

Microfluidic chip electrophoresis for biochemical analysis

Xiaowen Ou  1   2 , Peng Chen  2 , Xizhi Huang  2 , Shunji Li  2 , Bi-Feng Liu  2
Affiliations
Review

Microfluidic chip electrophoresis for biochemical analysis

Xiaowen Ou et al. J Sep Sci. 2020 Jan.

Abstract

Microfluidic chip electrophoresis has been widely employed for separation of various biochemical species owing to its advantages of low sample consumption, low cost, fast analysis, high throughput, and integration capability. In this article, we reviewed the development of four different modes of microfluidics-based electrophoresis technologies including capillary electrophoresis, gel electrophoresis, dielectrophoresis, and field (electric) flow fractionation. Coupling detection schemes on microfluidic electrophoresis platform were also reviewed such as optical, electrochemical, and mass spectrometry method. We further discussed the innovative applications of microfluidic electrophoresis for biomacromolecules (nucleic acids and proteins), biochemical small molecules (amino acids, metabolites, ions, etc.), and bioparticles (cells and pathogens) analysis. The future direction of microfluidic chip electrophoresis was predicted.

Keywords: analysis; biochemistry; electrophoresis; microfluidic chips.

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References

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