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. 2019 Nov-Dec;33(6):2265-2272.
doi: 10.21873/invivo.11732.

Level and Value of T Cell-derived Circulating Microparticles in Liver Cirrhosis Patients

Chih-Hung Chen  1 , Chia-Lo Chang  2 , Kuan-Hung Chen  3 , Ben-Chung Cheng  4 , Hong-Hwa Chen  2 , John Y Chiang  5   6 , Pei-Hsun Sung  7   8 , Hon-Kan Yip  7   8   9   10   11
Affiliations

Level and Value of T Cell-derived Circulating Microparticles in Liver Cirrhosis Patients

Chih-Hung Chen et al. In Vivo. 2019 Nov-Dec.

Abstract

Background/aim: We examined the hypothesis that T cell-derived-circulating microparticles (MPs) are increased in liver-cirrhosis (LC) patients compared to normal subjects and are also increased in chronic hepatitis compared to acute-decompensated-liver cirrhosis (ADLC).

Patients and methods: A total of 66 LC patients, including 35 with ADLC and 31 with non-decompensated-LC (NDLC), were enrolled in the study. Ten volunteers served as controls.

Results: Flow-cytometric analysis showed that circulating levels of T-cell derived MPs (i.e., total MPs and CD4+/CD8+/CD54+MPs) were higher in LC patients than in the controls (all p<0.003). Total MPs and CD8+MPs were higher in NDLC than in ADLC patients. There were good correlations between CD8+MPs and ADLC as well as between total MPs and chronic hepatitis. Multivariate-linear-regression analysis showed that NDLC was independently predictive of increased circulating CD8+MPs levels (p<0.05) and chronic hepatitis independently predictive of increased circulating total MPs levels (p<0.001)/CD4+MPs (p<0.05).

Conclusion: Circulating levels of T-cell-derived MPs were increased in ADLC patients and were even more elevated in NDLC patients compared to healthy-control subjects.

Keywords: Circulating T-cell derived microparticles; inflammation; liver cirrhosis.

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Conflict of interest statement

The Authors report no conflicts of interest regarding this study.

Figures

Figure 1
Figure 1. Flow chart illustrating the protocol of enrollment and allocation of study patients. ADLC: Acute decompensated liver cirrhosis; NDLC: non-decompensated liver cirrhosis.
Figure 2
Figure 2. Flow cytometric analysis of total circulating microparticles (MPs) among normal (n=10), ADLC (n=35), and NDLC (n=31) groups. The cirrhotic patients had a significant higher mean of total MPs than the normal population (p<0.001), whereas there was no significant difference in total MPs between ADLC and NDLC groups (p=0.253). Letters (a, b) were used for comparison of the same parameter among different groups, indicating significant difference (p<0.05) with one-way ANOVA. ADLC: Acute decompensated liver cirrhosis; NDLC: non-decompensated liver cirrhosis.
Figure 3
Figure 3. Flow cytometric analysis of individual circulating MPs among three groups. The mean values of circulating CD4+, CD8+ and CD54+ MPs were significantly higher in the cirrhotic than in the normal group (all p-values <0.002). However, the mean circulating MPs of CD11a+ and CD147+ didn’t differ between the cirrhotic and normal group. Additionally, all five individual circulating MPs also had no significant difference between the ADLC and NDLC groups. Letters (a, b) were used for comparison of the same parameter among different groups, indicating significant difference (p<0.05) with one-way ANOVA. ADLC: Acute decompensated liver cirrhosis; NDLC: non-decompensated liver cirrhosis.
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