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. 2018 Jan 27;55(1):60-69.
doi: 10.1515/helm-2017-0049. eCollection 2018 Mar.

Diplodiscus Mehrai Pande, 1937 and D. Japonicus (): Morphology of Developmental Stages and Molecular Data

V V Besprozvannykh  1 , K V Rozhkovan  1 , A V Ermolenko  1 , A V Izrailskaya  2
Affiliations

Diplodiscus Mehrai Pande, 1937 and D. Japonicus (): Morphology of Developmental Stages and Molecular Data

V V Besprozvannykh et al. Helminthologia. .

Abstract

Specimens of the snails Anisus centrifugops were infected with two types of cercariae relating to the genus Diplodiscus, differing from each other by body and organ sizes were found in a pond in the territory of Vladivostok (Primorsky region, Russia). Further study of their morphology and life cycles allowed us to establish that these flukes belong to Diplodiscus japonicus (Yamaguti, 1936) and D. mehrai Pande, 1937. Morphological and molecular data confirmed the validity of D. japonicus which had previously been synonymized with D. amphichrus Tubangui, 1933 and D. mehrai Pande, 1937.

Keywords: Diplodiscus japonicus; Diplodiscus mehrai; adult worm; cercariae; life cycle; metacercariae.

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Figures

Fig. 1
Fig. 1
Adult Diplodiscus: a – D. mehrai Pande, 1937, b – D. japonicus (Yamaguti, 1936).
Fig. 2
Fig. 2
Diplodiscus mehrai Pande, 1937: a – redia, b – cercaria, c and d – metacercaria, dorso-ventrally and laterally, accordingly; D. japonicus (Yamaguti, 1936): e – cercaria.
Fig. 3
Fig. 3
Diplodiscus mehrai Pande, 1937: а – adult worm, b – cercaria; D. japonicus (Yamaguti, 1936): c – adult worm, d – cercaria; e – metacercaria found in feces of snail.
Fig. 4
Fig. 4
Bayesian tree inferred from combined 18S and 28S rDNA sequence data. Results of statistical support are shown as follows: BI/ML. Sequences obtained in this study are in bold.
See this image and copyright information in PMC

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