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. 2020 Mar;51(1):403-407.
doi: 10.1007/s42770-019-00171-6. Epub 2019 Oct 29.

Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays

Dayanne A de Melo  1 , Bianca da S Soares  1 , Cássia C da Motta  1 , Felipe C Dubenczuck  1 , Nicolle L Barbieri  2 , Catherine M Logue  2 , Shana de O Coelho  1 , Irene da S Coelho  1 , Miliane M S de Souza  3
Affiliations

Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays

Dayanne A de Melo et al. Braz J Microbiol. 2020 Mar.

Abstract

Characterization of methicillin-resistant Staphylococcus (MRS) is a continuous challenge at diagnostic laboratories. The phenotypic methods present heterogeneous results and the occurrence of variants of mecA gene turned this goal even more challenging to achieve. The present study provided an accurate and highly discriminatory screening tool for MRS, improving its detection.

Keywords: Antimicrobial resistance; Methicillin-resistant Staphylococcus; mec variants; mecA genes.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Fig. 1
Fig. 1
Alignment of the mec genes sequences (KF058900, KF058901, KF058902 from variant mecA, and KF058903, KF058904, KF058905, KF058906, KF058907 from classical mecA). Gray areas represent the region of forward and reverse primers annealing
Fig. 2
Fig. 2
Amplification pattern of mec gene. (A) Universal (574 bp), (1) positive control, (2) blank, (3–6) positive strains. (B) Classical mecA (533 bp), (1) positive control, (2) blank, (3–6) positive strains. (C) Variant mecA (809 bp), (1) positive control, (2–5) positive strains, (6) blank. (D)mecC (718 bp), (1) blank, (2) positive control, (3–5) negative strains. MC, ladder (Hi-Lo DNA marker—10,000 bp)
See this image and copyright information in PMC

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