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. 2019 Oct 29;16(1):125.
doi: 10.1186/s12985-019-1222-9.

Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection

Chukiat Sirivichayakul  1 , Kriengsak Limkittikul  2 , Pornthep Chanthavanich  2 , Sutee Yoksan  3 , Anuttarasakdi Ratchatatat  4 , Jacqueline Kyungah Lim  5 , Watcharee Arunsodsai  2 , Arunee Sabchareon  2
Affiliations

Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection

Chukiat Sirivichayakul et al. Virol J. .

Abstract

Background: Dengue is an important mosquito-borne disease. There is currently only one licensed vaccine for dengue prevention. The vaccine provides higher efficacy in pre-vaccination dengue-seropositive persons but a higher risk of subsequent more severe dengue in dengue-seronegative persons. It is recommended that the dengue vaccine may be given in dengue-seropositive individuals or as mass vaccination without individual pre-vaccination screening in areas where the dengue seroprevalence is > 80% in children aged 9 years. We evaluated a dengue specific immunoglobulin G monoclonal antibody-based capture enzyme-linked immunosorbent assay (MAb-ELISA) in the diagnosis of previous dengue infection using serum samples from the cohort study in Ratchaburi Province, Thailand.

Methods: The MAb-ELISA was compared to 70% plaque reduction neutralization test (PRNT70) in 453 serum samples from children aged 3-11 years in Ratchaburi Province, Thailand.

Results: The sensitivity and specificity of MAb-ELISA at the positive to negative (P/N) ratio cut-off level of > 3 were both 0.91 in the diagnosis of previous dengue infection, compared to PRNT70. The false positivity was mainly in Japanese encephalitis (JE) seropositive subjects.

Conclusions: This research provides evidence that MAb-ELISA is useful for dengue seroprevalence study and dengue pre-vaccination screening. JE seropositivity was the major cause of false positive result in the study population.

Keywords: Dengue; Enzyme-linked immunosorbent assay; Monoclonal antibody; Plaque reduction neutralization test.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The receiver operating characteristic curve of MAb-ELISA compared to PRNT70. The green (lower) line represents the diagonal reference and the blue (upper) line indicates the performance of MAb-ELISA. With the blue line close to the top-left corner, the ROC curve supports that the MAb-ELISA is highly accurate, consistent with the results of the PRNT70
See this image and copyright information in PMC

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