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. 2019 Nov 1;9(1):20.
doi: 10.1186/s12348-019-0187-6.

Does ophthalmic-grade silicone oil possess antimicrobial properties?

Vivek Pravin Dave  1 , Joveeta Joseph  2 , Priyanka Jayabhasker  2 , Rajeev Reddy Pappuru  3 , Avinash Pathengay  4 , Taraprasad Das  3
Affiliations

Does ophthalmic-grade silicone oil possess antimicrobial properties?

Vivek Pravin Dave et al. J Ophthalmic Inflamm Infect. .

Abstract

Purpose: To test the antimicrobial properties of silicon oil (Aurosil 1000 cSt, Aurosil Plus 5000 cSt) on in vitro growth of common microorganisms causing endophthalmitis.

Materials and methods: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, multi-drug resistant (MDR) strain of Klebsiella pneumoniae, Escherichia coli, Candida albicans, and Aspergillus flavus were prepared to 0.5 McFarland turbidity. The bacteria and fungi were inoculated into the silicone oils, brain heart infusion (BHI) broth for bacteria and Sabouraud dextrose agar (SDA) broth for fungi, respectively, and cultured aerobically for 30 days. From each sample, 10 μl was plated onto nutrient agar and potato dextrose agar (PDA) for testing growth of bacteria and fungi respectively. Cultures from specimens, overnight incubation, and CFU counting were repeated on days 1, 3, 5, 7, 14, 21, 24, and 30. Negative controls were brain heart infusion and physiologic saline as well as silicone oils without any inoculations.

Results: All bacteria showed a decrease in CFUs by the fifth day and eliminated between 21 and 30 days in silicone oil. The silicon oil, irrespective of its viscosity, had only fungistatic effect up to 30 days. Colony-forming units of microorganisms remained stable in physiologic saline during the study. In BHI and Sabouraud broth, both bacteria and fungi showed a growth pattern that was compatible with the growth curve of microorganisms.

Conclusion: Medical-grade silicone oil used in ophthalmology exhibited in vitro bactericidal and fungistatic activity over 30 days. Insertion of silicone oil in vitrectomy for endophthalmitis, when required, could supplement the antimicrobial activities of intravitreal antibiotics in management of endophthalmitis.

Keywords: Antimicrobial activity; Endophthalmitis; Silicone oil.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Graph showing the growth pattern of Aspergillus. X-axis is in days, Y-axis has CFU (1 × 1010)
Fig. 2
Fig. 2
Graph showing the growth pattern of Candida albicans. X-axis is in days, Y-axis has CFU (1 × 1010)
Fig. 3
Fig. 3
Graph showing the growth pattern of Klebsiella pneumoniae. X-axis is in days, Y-axis has CFU (1 × 1010)
Fig. 4
Fig. 4
Graph showing the growth pattern of Pseudomonas aeruginosa. X-axis is in days, Y-axis has CFU (1 × 1010)
Fig. 5
Fig. 5
Graph showing the growth pattern of Staphylococcus aureus. X-axis is in days, Y-axis has CFU (1 × 1010)
Fig. 6
Fig. 6
Graph showing the growth pattern of Staphylococcus epidermidis. X-axis is in days, Y-axis has CFU (1 × 1010)
See this image and copyright information in PMC

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