Corrigendum to "Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells"

Abstract

[This corrects the article DOI: 10.1155/2016/8727289.].

Figure 1

Effect of the U0126 inhibitor on ERK and IκBα phosphorylation in RPMI 2650 cells stimulated with rhIL-17A. The cells were stimulated with rhIL-17A (20 ng/mL) or PMA (50 ng/mL) for 30 min in the absence or presence of U0126 (25 μM). (a) pERK and pIκBα protein expressions were evaluated in the cell lysates by western blot. The results were expressed as the ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of pERK, pIκBα, and β-actin are shown. The bands are spliced from uncropped blots. (b) The activation of ERK1/2 and NF-κB for each experimental condition was tested for the pERK1/2/total ERK1/2 ratio and for the pNF-κB/total NF-κB, respectively, by ELISA, and normalized for protein content. ANOVA with Fisher's test correction was used for the analysis of the data. p < 0.05 was statistically significant.

Figure 2

Effect of the U0126 inhibitor in RPMI 2650 cells stimulated with rhIL-17A. (a) The cells were stimulated with rhIL-17A (20 ng/mL) for 6 hrs in the absence or presence of U0126 (25 μM). ROS production was evaluated in the cells by flow cytometry. The bars represent the mean ± SD of 3 separate experiments. Representative flow cytometry is shown; (b) the cells were stimulated with rhIL-17A (20 ng/mL) for 18 hrs in the absence or presence of U0126 (25 μM). NOX-4 and IL-8 protein expressions were evaluated in the cell lysates by western blot. The results were expressed as the ratio of band intensity and β-actin of 3 separate experiments. Representative western blot is shown. The bands are spliced from uncropped blots. ANOVA with Fisher's test correction was used for the analysis of the data. p < 0.05 was statistically significant.

Figure 3

Effect of HMW-HA, MMW-HA, and LMW-HA on ERK1/2 and NF-κB signal pathway in RPMI 2650 cells stimulated with rhIL-17A. The cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 30 min; (a) pERK and pIκBα protein expressions were evaluated in the cell lysates by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of pERK, pIκBα, and β-actin are shown; (b) the activation of ERK1/2 and NF-κB for each experimental condition was tested for the pERK1/2/total ERK1/2 ratio and pNF-κB/total NF-κB ratio by ELISA and normalized for protein content. ANOVA with Fisher's test correction was used for the analysis of the data. p < 0.05 was statistically significant.

Figure 4

Effect of HMW-HA, MMW-HA, and LMW-HA in RPMI 2650 cells stimulated with rhIL-17A. (a) The cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 6 hrs. ROS production was evaluated in the cells by flow cytometry. The bars expressed the mean ± SD of 3 separate experiments. Representative flow cytometry is shown; (b) the cells were preincubated with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 18 hrs. NOX-4 and IL-8 protein syntheses were evaluated in the cell lysates by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative western blot is shown. ANOVA with Fisher's test correction was used for the analysis of the data. p < 0.05 was statistically significant.

Figure 5

Effect of U0126, HMW-HA, MMW-HA, and LMW-HA on nuclear translocation of NF-κB in RPMI 2650 cells stimulated with rhIL-17A. The cells were preincubated (a) with U0126 (25 μM) or (b) with HMW-HA (100 μg/mL), MMW-HA (100 μg/mL), and LMW-HA (100 μg/mL) for 1 h and then stimulated with rhIL-17A (20 ng/mL) for 30 min; NF-κB was evaluated in nuclear cell lysate by western blot. The bars represent the ratio of band intensity and β-actin of 3 separate experiments. Representative gel images of NF-κB and β-actin are shown. ANOVA with Fisher's test correction was used for the analysis of the data. p < 0.05 was statistically significant.