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. 2019 Oct 23;5(10):e02637.
doi: 10.1016/j.heliyon.2019.e02637. eCollection 2019 Oct.

New spectral resolution techniques for resolving and determining the components in binary fixed-dose combinations

Duaa J Al Zakri  1 , Reem H Obaydo  1 , Amir Alhaj Sakur  1
Affiliations

New spectral resolution techniques for resolving and determining the components in binary fixed-dose combinations

Duaa J Al Zakri et al. Heliyon. .

Abstract

Four spectrophotometric approaches were performed to determine a binary combination of Phenazone and Benzocaine in pure powder form and in pharmaceutical formations. This investigation submits the application of four techniques contingent on the presence of the extended area of the spectra of one compound in the binary mixture, these methods include Absorptivity Centering (a-centering), Absorbance Subtraction (AS), Amplitude Modulation (AM) and Concentration Value (CV). The linearity range for the above-mentioned approaches was found to be 3.0-15.0 μg/mL for Benzocaine in a-centering method and 3.0-30.0 μg/mL for Benzocaine and Phenazone in other advanced methods. The four techniques were evaluated as per ICH criteria and were successfully utilized for the determination of Phenazone and Benzocaine existing in pharmaceutical formulations. All results gained by the submitted approaches were statistically compared with a previously published method, and no important differences were detected.

Keywords: Analytical chemistry; Pharmaceutical chemistry.

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Figures

Fig. 1
Fig. 1
Structural formulae of (a) Phenazone and (b) Benzocaine.
Fig. 2
Fig. 2
Zero-order spectra of [PHN] 13.5 μg/mL and [BEN] 3.5 μg/mL in ethanol.
Fig. 3
Fig. 3
Zero-order spectra of mixtures of PHN + BEN, with a total concentration equal to 10.0 μg/mL showing the three iso-absorptive points.
Fig. 4
Fig. 4
Ratio spectra of 10.0 μg/mL of PHN, BEN separately in ethanol and their binary mixture,10.0 μg/mL of each in ethanol. using the (NSʹ BEN) as a divisor showing the constant region.
Fig. 5
Fig. 5
The constant value gained by dividing the zero order spectra of [BEN] (3.0–30.0 μg/mL) by the normalized spectra (1.0 μg/mL of BEN).
Fig. 6
Fig. 6
The constant value gained by dividing the zero order spectra of [PHN] (3.0–30.0 μg/mL) by the normalized spectra (1.0 μg/mL of PHN).
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References

    1. Martindale Sweetman. 33rd ed. Pharmaceutical Press; UK: 2002. The Complete Drug References.
    1. Arale S.S., Phoujdar M.S., Vassa S.P. UV spectrophotometric estimation of phenazone in bulk and tablet dosage form by Auc method. WJPPS. 2014;3(8):1734–1741.
    1. Ribone M.É., Pagani A.P., Olivieri A.C. Simultaneous multivariate spectrophotometric analysis of ear drops containing a ternary mixture of antipyrine, sulfathiazole, and rivanol. Anal. Lett. Aug. 2001;34(12):2077–2088.
    1. Collado M.S., Mantovani V.E., Goicoechea H.C., Olivieri A.C. Simultaneous spectrophotometric-multivariate calibration determination of several components of ophthalmic solutions: phenylephrine, chloramphenicol, antipyrine, methylparaben and thimerosal. Talanta. Aug. 2000;52(5):909–920. - PubMed
    1. Santoni G., Mura P., Pinzauti S., Lombardo E., Gratteri P. Simultaneous UV spectrophotometric determination of procaine hydrochloride and phenazone in an otic formulation. Int. J. Pharm. Oct. 1990;64(2–3):235–238.