Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Jun;7(6):1845-52.
doi: 10.1002/j.1460-2075.1988.tb03017.x.

The functional significance of DNA sequence structure in a site-specific genetic recombination reaction

S W Umlauf  1 , M M Cox
Affiliations

The functional significance of DNA sequence structure in a site-specific genetic recombination reaction

S W Umlauf et al. EMBO J. 1988 Jun.

Abstract

A series of sequence changes in the spacer region of the FLP recombination target (FRT) site are presented which drastically reduce site function without affecting recognition by the FLP protein. The effects follow a pattern which indicates that two structural features of the FRT site are essential for site function: a pair of pyrimidine tracts arranged in a palindrome and a predominance of AT base pairs in the spacer. The FRT site represents a sequence that serves to facilitate unwinding of the DNA within the spacer region during recombination. The results provide a clear demonstration of a role for a DNA sequence element that is distinct from protein recognition.

PubMed Disclaimer

References

    1. Cell. 1986 Aug 15;46(4):541-50 - PubMed
    1. Nature. 1979 Dec 13;282(5740):680-6 - PubMed
    1. EMBO J. 1985 Jul;4(7):1839-45 - PubMed
    1. Proc R Soc Lond B Biol Sci. 1981 Nov 24;213(1193):479-87 - PubMed
    1. J Biol Chem. 1987 Sep 25;262(27):13188-97 - PubMed

Publication types

MeSH terms