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. 2019 Nov;9(11):411.
doi: 10.1007/s13205-019-1949-4. Epub 2019 Oct 22.

Lipase production from mutagenic strain of Fusarium Incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation

Ritika Joshi #  1 , Rekha Sharma #  2 , Rupam Bhunia  3 , Anand Prakash  1 , Arindam Kuila  1
Affiliations

Lipase production from mutagenic strain of Fusarium Incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil degradation

Ritika Joshi et al. 3 Biotech. 2019 Nov.

Abstract

In the present study, lipase production from mutated strain of Fusarium incarnatum KU377454 was optimized through central composite design (CCD) based response surface methodology (RSM). The maximum lipase production (4.01 IU/mL) was obtained within 4 days of incubation using 0.1% CaCl2 concentration and 8% wheat bran concentration. Further, salting out technique was applied for partial purification of lipase. The partially purified lipase was immobilized using Au@Ag bimetallic nanoshell. The characterization of immobilized lipase was carried out by transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), Fourier transformed infrared (FTIR), energy dispersive X-ray (EDX), X-ray diffraction (XRD) and thermo gravimetric analysis (TGA). The immobilized lipase could retain its 95% of activity after 15 days of storage at 4 °C. Subsequently, Au@Ag immobilized lipase was used for the degradation of waste cooking oil (WCO), which showed higher WCO degradation (85%) compared to the free lipase mediated waste cooking oil degradation (71%). The immobilized lipase could be reused for five times without any loss of its activity.

Keywords: Au–Ag core shells; Characterization; Immobilization; Lipase; Optimization; WCO degradation.

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Conflict of interest statement

Conflict of interestThe authors declare that they don’t have any conflict of interest in the publication.

Figures

Fig. 1
Fig. 1
RSM plot showing the effect of yeast extract concentration and incubation time on lipase production
Fig. 2
Fig. 2
RSM plot showing the effect of CaCl2 concentration and yeast extract concentration on lipase production
Fig. 3
Fig. 3
RSM plot showing the effect of substrate concentration and yeast extract concentration on lipase production
Fig. 4
Fig. 4
Effect of pH on stability of lipase (free and immobilized)
Fig. 5
Fig. 5
Effect of temeprature on stability of lipase (free and immobilized)
Fig. 6
Fig. 6
a UV–Visible spectrum of (i) AgNPs (ii) AuNPs (iii) Au@Ag NPs and b Optical image of gold sol, AgNPs, and Au@Ag NPs
Fig. 7
Fig. 7
a SEM image of AuNPs b Au@Ag NPs c TEM image, and d SAED pattern of Au@Ag NPs
Fig. 8
Fig. 8
a EDX spectrum of Au@Ag NPs b corresponding elemental mapping of Au@Ag NPs
Fig. 9
Fig. 9
XRD spectrum analysis of Au@Ag NPs
Fig. 10
Fig. 10
FTIR spectrum analysis of a AgNPs and b Au@Ag NPs
Fig. 11
Fig. 11
a TGA and b DTG spectrum analysis of Au@Ag NPs
Fig. 12
Fig. 12
Effect of incubation time on waste cooking oil conversion by immobilized and free lipase
Fig. 13
Fig. 13
Reusability of nanoparticle immobilized lipase for waste cooking oil conversion
See this image and copyright information in PMC

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