Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Oct 18;7(1):31-34.
doi: 10.1080/23144599.2019.1675287. eCollection 2019.

Brain aromatase modulates cardiac functions in embryonic zebrafish

Zulvikar Syambani Ulhaq  1
Affiliations

Brain aromatase modulates cardiac functions in embryonic zebrafish

Zulvikar Syambani Ulhaq. Int J Vet Sci Med. .

Abstract

Oestradiol (E2) is known as a female reproductive hormone with pleiotropic effects on the cardiovascular system. Local E2 biosynthesis such as in the brain and myocardial cells have important physiological and pathophysiological roles. E2 production is catalysed by aromatase (Aro) enzyme. In teleost, two Aro isoforms are distinctly expressed in the ovary and brain. In this study, the role of brain Aro (AroB) in modulating cardiovascular system is investigated. AroB MO-mediated knockdown decreased ventricular functions. Moreover, embryos injected with AroB MO displays a sign in developing heart failure. All the effects caused by AroB MO were partially reversed by exposure to E2. Taken together, this study demonstrates the role of AroB in modulating normal cardiovascular function in zebrafish embryos.

Keywords: Brain aromatase; cardiovascular; oestradiol; zebrafish.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
AroB MO-mediated effects on cardiac functions. (a) Representative images of a lateral view of heart region of 48-hpf embryos (uninjected control (C), InvB MO-injected and AroB MO-injected embryos, and E2 exposed embryos). (a) Atrium; V, Ventricle; L, Length; W, Width. (b) Measurement of heart ventricle at diastolic and systolic phase. Width of ventricle (a); length of ventricle (b); cross-sectional area of ventricle (c). (c) Measurement of EDV, ESV and SV. (d) Measurement of FS, FAC and EF. (e) Measurement of CO. Data are presented as a mean ± SEM. (*) indicates a significant difference compared to control (p < 0.05). Ns, not significant; EDV, end-diastolic volume; ESV, end-systolic volume; SV, stroke volume; FS, fractional shortening; FAC, fractional area change; EF, ejection fraction; and CO, cardiac output.
Figure 2.
Figure 2.
E2 reversed the effects of AroB MO-mediated knockdown on cardiac functions. (a) Measurement of heart ventricle at diastolic and systolic phase. Width of ventricle (a); length of ventricle (b); cross-sectional area of ventricle (c). (b) Measurement of EDV, ESV and SV. (c) Measurement of FS, FAC and EF. (d) Measurement of CO. Data are presented as a mean ± SEM. (*) indicates a significant difference compared to control (p < 0.05).
See this image and copyright information in PMC

References

    1. Simpson ER, Davis SR.. Minireview: aromatase and the regulation of estrogen biosynthesis-some new perspectives. Endocrinology. 2001;142:4589–4594. - PubMed
    1. Kishida M, Callard GV. Distinct cytochrome P450 aromatase isoforms in zebrafish (Danio rerio) brain and ovary are differentially programmed and estrogen regulated during early development. Endocrinology. 2001;142:740–750. - PubMed
    1. Ulhaq ZS, Kishida M. Brain aromatase modulates serotonergic neuron by regulating serotonin levels in zebrafish embryos and larvae. Front Endocrinol. 2018;9:230. - PMC - PubMed
    1. Allgood OE Jr, Hamad A, Fox J, et al. Estrogen prevents cardiac and vascular failure in the ‘listless’ zebrafish (Danio rerio) developmental model. Gen Comp Endocrinol. 2013;189:33–42. - PubMed
    1. Jazbutyte V, Stumpner J, Redel A, et al. Aromatase inhibition attenuates desflurane-induced preconditioning against acute myocardial infarction in male mouse heart in vivo. PLoS ONE. 2012;7:e42032. - PMC - PubMed

LinkOut - more resources