Effects of abomasal infusion of essential fatty acids and conjugated linoleic acid on performance and fatty acid, antioxidative, and inflammatory status in dairy cows

Abstract

The objective of this study was to test the effects of essential fatty acids (EFA), particularly α-linolenic acid, and conjugated linoleic acid (CLA) supplementation on fatty acid (FA) composition, performance, and systemic and hepatic antioxidative and inflammatory responses in dairy cows. Four cows (126 ± 4 d in milk) were investigated in a 4 × 4 Latin square and were abomasally infused with 1 of the following for 6 wk: (1) coconut oil (control treatment, CTRL; 38.3 g/d; providing saturated FA), (2) linseed and safflower oil (EFA treatment; 39.1 and 1.6 g/d, respectively; providing mainly α-linolenic acid), (3) Lutalin (BASF, Ludwigshafen, Germany; CLA treatment; cis-9,trans-11 and trans-10,cis-12 CLA, 4.6 g/d each), (4) or EFA+CLA. The initial dosage was doubled every 2 wk, resulting in 3 dosages (dosage 1, 2, and 3). Cows were fed a corn silage-based total mixed ration with a high n-6/n-3 FA ratio. Dry matter intake and milk yield were recorded daily, and milk composition was measured weekly. The FA compositions of milk fat and blood plasma were analyzed at wk 0, 2, 4, and 6. The plasma concentration and hepatic mRNA abundance of parameters linked to the antioxidative and inflammatory response were analyzed at wk 0 and 6 of each treatment period. Infused FA increased in blood plasma and milk of the respective treatment groups in a dose-dependent manner. The n-6/n-3 FA ratio in milk fat was higher in CTRL and CLA than in EFA and EFA+CLA. The sum of FA <C16 in milk fat decreased in CLA and EFA+CLA in a dosage-dependent manner. Energy-corrected milk and milk fat decreased in CLA and EFA+CLA in a dosage-dependent manner and were higher in EFA and CTRL than in CLA at dosages 2 and 3. Energy balance tended to be highest in CLA cows. Milk protein content was lower in CLA and EFA+CLA than in CTRL. Milk urea concentration decreased in CLA and EFA+CLA in a dosage-dependent manner and was lower in CLA and EFA+CLA than in EFA and CTRL at dosages 2 and 3. Milk citrate concentration increased in CLA in a dosage-dependent manner and was higher in CLA and EFA+CLA than in EFA and CTRL. Glutathione peroxidase activity in blood plasma was lower in CTRL than in EFA, and plasma concentration of β-carotene increased in EFA and EFA+CLA with dosage. Increased milk citrate pointed at reduced de novo FA synthesis and a better antioxidative status in milk due to CLA treatment. Supplementation with CLA may also affect milk protein synthesis, but EFA and CLA treatment did not influence the inflammatory status in a consistent manner in mid-lactating cows.

Keywords: conjugated linoleic acid; dairy cow; essential fatty acid; inflammatory status.