Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Sep;24(9):959-62.
doi: 10.1007/BF02623911.

Production of human-human hybridomas secreting monoclonal antibodies reactive to breast cancer cell lines

K Aihara  1 , K Yamada, H Murakami, Y Nomura, H Omura
Affiliations

Production of human-human hybridomas secreting monoclonal antibodies reactive to breast cancer cell lines

K Aihara et al. In Vitro Cell Dev Biol. 1988 Sep.

Abstract

We produced human-human hybridomas by fusing lymph node lymphocytes of breast cancer patients with a fusion partner, HO-323 cells, in the presence of 50% polyethylene glycol, and screened hybridomas producing monoclonal antibody (MoAb) reactive to a breast cancer cell line, MCF-7. Among 11 hybridomas secreting IgM reactive to MCF-7, four hybridomas (H15A3, H15F2, K15B4, and K15C3) produced IgM reacting specifically to breast cancer cell lines (MCF-7 and HBC-5). Among them, H15A3 proliferated in a serum-free (RDF) medium supplemented with insulin, transferrin, ethanolamine, selenium, and endothelial cell growth supplement, as fast as in 10% FCS-RDF medium. According to transfer blotting analysis, the H15F2 MoAb reacted with a 40,000 dalton antigen in MCF-7 cells.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1332-6 - PubMed
    1. J Natl Cancer Inst. 1983 Mar;70(3):409-19 - PubMed
    1. Biotechnol Bioeng. 1988 Feb 5;31(2):168-72 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1158-62 - PubMed

LinkOut - more resources