Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2019 Dec;20(12):719-727.
doi: 10.1038/s41583-019-0231-4. Epub 2019 Nov 8.

Optical voltage imaging in neurons: moving from technology development to practical tool

Thomas Knöpfel  1 , Chenchen Song  2
Affiliations
Review

Optical voltage imaging in neurons: moving from technology development to practical tool

Thomas Knöpfel et al. Nat Rev Neurosci. 2019 Dec.

Abstract

A central goal in neuroscience is to determine how the brain's neuronal circuits generate perception, cognition and emotions and how these lead to appropriate behavioural actions. A methodological platform based on genetically encoded voltage indicators (GEVIs) that enables the monitoring of large-scale circuit dynamics has brought us closer to this ambitious goal. This Review provides an update on the current state of the art and the prospects of emerging optical GEVI imaging technologies.

PubMed Disclaimer

References

    1. Markram, H. et al. Reconstruction and simulation of neocortical microcircuitry. Cell 163, 456–492 (2015). - PubMed - PMC
    1. Seeman, S. C. et al. Sparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex. eLife 7, e37349 (2018). - PubMed - PMC
    1. Knöpfel, T. Genetically encoded optical indicators for the analysis of neuronal circuits. Nat. Rev. Neurosci. 13, 687–700 (2012). - PubMed
    1. Knöpfel, T., Diez-Garcia, J. & Akemann, W. Optical probing of neuronal circuit dynamics: genetically encoded versus classical fluorescent sensors. Trends Neurosci. 29, 160–166 (2006). This study is an early account of the potential of genetically encoded indicators, with arguments that have become common sense over the past few years. - PubMed
    1. Scanziani, M. & Hausser, M. Electrophysiology in the age of light. Nature 461, 930–939 (2009). This article provides an appraisal of then-emerging optical methods. - PubMed

Publication types

MeSH terms

LinkOut - more resources