doi: 10.1016/0091-6749(88)90977-3.
Evaluation of a radioimmunoassay for histamine measurement in biologic fluids
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- PMID: 3171005
- DOI: 10.1016/0091-6749(88)90977-3
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Evaluation of a radioimmunoassay for histamine measurement in biologic fluids
J Allergy Clin Immunol.
1988 Oct.
Abstract
A new radioimmunoassay for the measurement of histamine in biologic fluids was evaluated. Assay selectivity and specificity were achieved by "succinyl-glycinamide derivatization" of histamine in samples to mimic the immunogen used to generate the monoclonal antibody. The assay exhibits a linear response from 0.1 to 5.0 ng/ml of histamine and the monoclonal antibody used has partial recognition of only N-methylhistamine (other than histamine). With minimal modifications, the assay can accurately measure histamine in plasma, urine, and buffer. Normal ranges for human subjects were established: plasma levels are 0.193 +/- 0.08 ng/ml (n = 40) and urine levels are 20.9 +/- 11.2 micrograms histamine/gm creatinine (n = 10).
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